通过MSQPCR对同时采集的室内和室外空气中霉菌浓度进行的比较。
Comparison of mold concentrations quantified by MSQPCR in indoor and outdoor air sampled simultaneously.
作者信息
Meklin Teija, Reponen Tiina, McKinstry Craig, Cho Seung-Hyun, Grinshpun Sergey A, Nevalainen Aino, Vepsäläinen Asko, Haugland Richard A, Lemasters Grace, Vesper Stephen J
机构信息
National Public Health Institute, Finland.
出版信息
Sci Total Environ. 2007 Aug 15;382(1):130-4. doi: 10.1016/j.scitotenv.2007.03.031. Epub 2007 Apr 30.
Abstract
Mold specific quantitative PCR (MSQPCR) was used to measure the concentrations of the 36 mold species in indoor and outdoor air samples that were taken simultaneously for 48 h in and around 17 homes in Cincinnati, Ohio. The total spore concentrations of 353 per m(3) of indoor air and 827 per m(3) of outdoor air samples were significantly different (p<or=0.05). However, only the concentrations of Aspergillus penicillioides, Cladosporium cladosporioides types 1 and 2 and Cladosporium herbarum were correlated in indoor and outdoor air samples (p-value<or=0.05 and sufficient data for estimate and absolute value rho estimate >or=0.5). These results suggest that interpretation of the meaning of short-term (<48 h) mold measurements in indoor and outdoor air samples must be made with caution.
摘要
采用霉菌特异性定量聚合酶链反应（MSQPCR）来测定俄亥俄州辛辛那提市17所房屋及其周边室内和室外空气样本中36种霉菌的浓度，这些样本同时采集48小时。室内空气样本每立方米353个孢子的总孢子浓度与室外空气样本每立方米827个孢子的总孢子浓度存在显著差异（p≤0.05）。然而，仅室内和室外空气样本中，青霉状曲霉、枝孢枝孢1型和2型以及草本枝孢的浓度具有相关性（p值≤0.05，且有足够数据进行估计且绝对值相关系数估计值≥0.5）。这些结果表明，对于室内和室外空气样本中短期（<48小时）霉菌测量结果的解读必须谨慎。
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