Wang Baohua, Guo Wangzhen, Zhu Xiefei, Wu Yaoting, Huang Naitai, Zhang Tianzhen
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China.
J Genet Genomics. 2007 Jan;34(1):35-45. doi: 10.1016/S1673-8527(07)60005-8.
A population of 180 recombinant inbred lines (RILs) was developed by single seed descended from the cross of high yield Upland cotton (Gossypium hirsutum L.) varieties Zhongmiansuo12 (ZMS12) and 8891, the two parents of Xiangzamian2 (XZM2). A genetic linkage map consisting of 132 loci and covering 865.20 cM was constructed using the RIL population chiefly with SSR markers. Yield and yield components were investigated for RILs in three environments in China. The purpose of the present research was to analyze the relationship between yield and its components and to map QTL for yield and yield components in cotton. QTL were tagged with data sets from single environment (separate analysis) and a set of data from means of the three environments (joint analysis). A total of 34 QTL for yield and yield components were independently detected in three environments, whereas fifteen QTL were found in joint analysis. Notably, a stable lint percentage QTL qLP-A10-1 was detected both in joint analysis and in two environments of separate analysis, which might be of special value for marker-assisted selection. The QTL detected in the present study provide new information on improving yield and yield components. Results of path analysis showed that bolls/plant had the largest contribution to lint yield, which is consistent with the mid-parent heterosis value in F(1). Accordingly, in cotton breeding, bolls/plant can be considered first and other yield components measured as a whole to implement variety enhancement and hybrid selection of cotton.
以高产陆地棉（Gossypium hirsutum L.）品种中棉所12（ZMS12）和8891（湘杂棉2号（XZM2）的两个亲本）杂交产生的单粒传后代构建了一个包含180个重组自交系（RIL）的群体。主要利用SSR标记，以该RIL群体构建了一张由132个位点组成、覆盖865.20 cM的遗传连锁图谱。在中国的三个环境中对RIL的产量及其构成因素进行了调查。本研究的目的是分析产量与其构成因素之间的关系，并定位棉花产量及其构成因素的QTL。用来自单一环境的数据集（单独分析）和来自三个环境均值的一组数据集（联合分析）对QTL进行定位。在三个环境中独立检测到34个产量及其构成因素的QTL，而在联合分析中发现了15个QTL。值得注意的是，在联合分析以及单独分析的两个环境中均检测到一个稳定的皮棉率QTL qLP-A10-1，这可能对标记辅助选择具有特殊价值。本研究中检测到的QTL为提高产量及其构成因素提供了新信息。通径分析结果表明，单株铃数对皮棉产量的贡献最大，这与F(1)中的中亲杂种优势值一致。因此，在棉花育种中，可以首先考虑单株铃数，将其他产量构成因素作为一个整体来衡量，以实现棉花品种改良和杂交选择。
