Nakamura Yoshimasa, Yoshimoto Motoko, Murata Yoshiyuki, Shimoishi Yasuaki, Asai Yumi, Park Eun Young, Sato Kenji, Nakamura Yasushi
Department of Biofunctional Chemistry, Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
J Agric Food Chem. 2007 May 30;55(11):4407-13. doi: 10.1021/jf070159w. Epub 2007 May 1.
In the present study, papaya (Carica papaya) seed and edible pulp were carefully separated and then the contents of benzyl isothiocyanate and the corresponding glucosinolate (benzyl glucosinolate, glucotropaeolin) quantified in each part. The papaya seed with myrosinase inactivation contained >1 mmol of benzyl glucosinolate in 100 g of fresh seed. This content is equivalent to that of Karami daikon (the hottest Japanese white radish) or that of cress. The papaya seed extract also showed a very high activity of myrosinase and, without myrosinase inactivation, produced 460 micromol of benzyl isothiocyanate in 100 g of seed. In contrast, papaya pulp contained an undetectable amount of benzyl glucosinolate and showed no significant myrosinase activity. The n-hexane extract of the papaya seed homogenate was highly effective in inhibiting superoxide generation and apoptosis induction in HL-60 cells, the activities of which are comparable to those of authentic benzyl isothiocyanate.
在本研究中，仔细分离了木瓜（番木瓜）种子和可食用果肉，然后对各部分中苄基异硫氰酸酯和相应的硫代葡萄糖苷（苄基硫代葡萄糖苷，黑芥子硫苷酸钾）含量进行了定量分析。失活了黑芥子酶的木瓜种子在100克新鲜种子中含有>1毫摩尔的苄基硫代葡萄糖苷。这一含量与卡拉米萝卜（最辣的日本白萝卜）或水田芥相当。木瓜种子提取物还显示出非常高的黑芥子酶活性，在未失活黑芥子酶的情况下，100克种子可产生460微摩尔的苄基异硫氰酸酯。相比之下，木瓜果肉中苄基硫代葡萄糖苷含量未检出，且未显示出明显的黑芥子酶活性。木瓜种子匀浆的正己烷提取物在抑制HL-60细胞中超氧化物生成和凋亡诱导方面非常有效，其活性与正宗的苄基异硫氰酸酯相当。
