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Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization.通过荧光原位杂交快速鉴定临床相关肠球菌种类
J Clin Microbiol. 2007 Oct;45(10):3424-6. doi: 10.1128/JCM.00861-07. Epub 2007 Aug 1.
2
Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay.采用改良荧光原位杂交分析法快速鉴定血培养中的病原体
J Clin Microbiol. 2006 Nov;44(11):4186-8. doi: 10.1128/JCM.01085-06.
3
Rapid emergence of resistance to linezolid during linezolid therapy of an Enterococcus faecium infection.在粪肠球菌感染的利奈唑胺治疗期间，对利奈唑胺的耐药性迅速出现。
Antimicrob Agents Chemother. 2006 Dec;50(12):4217-9. doi: 10.1128/AAC.00518-06. Epub 2006 Sep 18.
4
Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes.使用掺入锁核酸的DNA探针提高原位杂交效率。
Appl Environ Microbiol. 2006 Aug;72(8):5311-7. doi: 10.1128/AEM.03039-05.
5
Characterization of mononucleotide repeats in sequenced prokaryotic genomes.已测序原核生物基因组中单核甘酸重复序列的特征分析
DNA Res. 2005;12(4):221-33. doi: 10.1093/dnares/dsi009.
6
Central venous catheter colonization by linezolid-resistant, vancomycin-susceptible Enterococcus faecalis.耐利奈唑胺、万古霉素敏感的粪肠球菌引起的中心静脉导管定植
J Clin Microbiol. 2006 May;44(5):1915-6. doi: 10.1128/JCM.44.5.1915-1916.2006.
7
Emergence of linezolid resistance in the vancomycin-resistant Enterococcus faecium multilocus sequence typing C1 epidemic lineage.耐万古霉素屎肠球菌多位点序列分型C1流行谱系中利奈唑胺耐药性的出现。
J Clin Microbiol. 2006 Mar;44(3):1153-5. doi: 10.1128/JCM.44.3.1153-1155.2006.
8
Comparison of testing methods for detection of decreased linezolid susceptibility due to G2576T mutation of the 23S rRNA gene in Enterococcus faecium and Enterococcus faecalis.粪肠球菌和屎肠球菌中因23S rRNA基因G2576T突变导致的利奈唑胺敏感性降低的检测方法比较
J Clin Microbiol. 2006 Mar;44(3):1098-100. doi: 10.1128/JCM.44.3.1098-1100.2006.
9
Emergence of linezolid resistance in Enterococcus faecium not dependent on linezolid treatment.粪肠球菌中利奈唑胺耐药性的出现不依赖于利奈唑胺治疗。
Eur J Clin Microbiol Infect Dis. 2006 Mar;25(3):197-8. doi: 10.1007/s10096-006-0100-8.
10
Trends in linezolid susceptibility patterns: report from the 2002-2003 worldwide Zyvox Annual Appraisal of Potency and Spectrum (ZAAPS) Program.利奈唑胺药敏模式趋势：2002 - 2003年全球Zyvox年度效力与谱评估（ZAAPS）项目报告
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通过荧光原位杂交检测肠球菌属中对利奈唑胺耐药的突变

Detection of mutations conferring resistance to linezolid in Enterococcus spp. by fluorescence in situ hybridization.

作者信息

Werner Guido, Bartel Melanie, Wellinghausen Nele, Essig Andreas, Klare Ingo, Witte Wolfgang, Poppert Sven

机构信息

Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Kochstr. 8, D-89081 Ulm, Germany.

出版信息

J Clin Microbiol. 2007 Oct;45(10):3421-3. doi: 10.1128/JCM.00179-07. Epub 2007 May 2.

DOI:10.1128/JCM.00179-07
PMID:17475756
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2045332/
Abstract

A fluorescence in situ hybridization (FISH) assay was established to detect linezolid resistance (conferred by the mutation 2576G>T in the gene coding for the 23 string of the ribosomal RNA) in enterococci. The assay was evaluated with 106 Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify a single mutated allele in phenotypically linezolid-susceptible isolates.

摘要

建立了一种荧光原位杂交（FISH）检测方法，用于检测肠球菌对利奈唑胺的耐药性（由核糖体RNA 23链编码基因中的2576G>T突变引起）。用106株肠球菌分离株对该检测方法进行了评估；结果显示，检测表型耐药性的灵敏度为100%，甚至能够在表型对利奈唑胺敏感的分离株中鉴定出单个突变等位基因。