Oursel Delphine, Loutelier-Bourhis Corinne, Orange Nicole, Chevalier Sylvie, Norris Victor, Lange Catherine M
Spectrométrie de masse Bio-Organique, CNRS UMR 6014, Université de Rouen, 76821 Mont-saint-Aignan cedex, France.
Rapid Commun Mass Spectrom. 2007;21(20):3229-33. doi: 10.1002/rcm.3177.
The lipids that are essential to the functioning of the bacterial membrane exist in hundreds of different forms. The reasons for this diversity are far from clear but are presumably related to the roles of these lipids in both facilitating enzymic activities and generating proteolipid domains. A full understanding of bacterial physiology therefore requires characterization of lipids in different strains in a variety of environmental conditions. This characterization then becomes the basis for lipidomics, the lipid aspect of the growing field of metabolomics. To exploit the power of derivatization chemistry and of gas chromatography/mass spectrometry (GC/MS) and tandem mass spectrometry (MS/MS) for metabolomics studies, we report here the development of various GC/MS electron ionization (EI) and negative and positive chemical ionization (CI) methods for the identification and, for the first time, the relative quantification of fatty acids present in extracts from membranes of a laboratory strain of Escherichia coli. They consist of seven saturated fatty acids (C10:0, C12:0, C14:0, C15:0, C16:0, C17:0 and C18:0) and six unsaturated fatty acids (C16:1, cyC17:0 plus two isomers of C18:1, C18:2 and cyC19:0).
对细菌膜功能至关重要的脂质存在数百种不同形式。这种多样性的原因尚不清楚，但可能与这些脂质在促进酶活性和形成蛋白脂质结构域方面的作用有关。因此，全面了解细菌生理学需要在各种环境条件下对不同菌株中的脂质进行表征。这种表征随后成为脂质组学的基础，脂质组学是不断发展的代谢组学领域的脂质方面。为了利用衍生化化学以及气相色谱/质谱联用（GC/MS）和串联质谱（MS/MS）在代谢组学研究中的强大功能，我们在此报告开发了各种GC/MS电子电离（EI）以及正负化学电离（CI）方法，用于鉴定并首次相对定量实验室菌株大肠杆菌膜提取物中存在的脂肪酸。它们包括七种饱和脂肪酸（C10:0、C12:0、C14:0、C15:0、C16:0、C17:0和C18:0）和六种不饱和脂肪酸（C16:1、环C17:0以及C18:1的两种异构体、C18:2和环C19:0）。
