Zhao Z W, Chen X J, Tay B K, Chen J S, Han Z J, Khor K A
School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore.
Biosens Bioelectron. 2007 Aug 30;23(1):135-9. doi: 10.1016/j.bios.2007.03.014. Epub 2007 Mar 30.
ZnO:Co nanoclusters were synthesized by nanocluster-beam deposition with averaged particle size of 5 nm and porous structure, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized into the ZnO:Co nanocluster-assembled thin film through Nafion-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of the ZnO:Co nanoclusters, the constructed glucose biosensor showed a high sensitivity of 13.3 microA/mA cm2. The low detection limit was estimated to be 20 microM (S/N=3) and the apparent Michaelis-Menten constant was found to be 21 mM, indicating the high affinity of the enzyme on ZnO:Co nanoclusters to glucose. The results show that the ZnO:Co nanocluster-assembled thin films with nanoporous structure and nanocrystallites have potential applications as platforms to immobilize enzyme in biosensors.
通过纳米团簇束沉积法合成了平均粒径为5 nm且具有多孔结构的ZnO:Co纳米团簇，首次采用这些纳米团簇构建了新型安培型葡萄糖生物传感器。通过Nafion辅助交联技术将葡萄糖氧化酶固定在ZnO:Co纳米团簇组装薄膜中。由于ZnO:Co纳米团簇具有高比活性位点和高电催化活性，构建的葡萄糖生物传感器显示出13.3 μA/mA cm2的高灵敏度。估计检测限低至20 μM（S/N = 3），表观米氏常数为21 mM，表明该酶对ZnO:Co纳米团簇上的葡萄糖具有高亲和力。结果表明，具有纳米多孔结构和纳米微晶的ZnO:Co纳米团簇组装薄膜作为生物传感器中固定酶的平台具有潜在应用。
