Markesbery W R, Brooks W H, Milsow L, Mortara R H
Cancer. 1976 Jan;37(1):327-37. doi: 10.1002/1097-0142(197601)37:1<327::aid-cncr2820370143>3.0.co;2-4.
The fine structure of a pineal germinoma was studied in tissue blocks and in tissue culture. The original tumor contained large primary tumor cells, lymphocytes, macrophages, plasma cells, and astrocytes. The large primary tumor cells were characterized by large nuclei, relatively scanty organelles, abundant glycogen, and junctional complexes. The neoplasm grew well in vitro and, with the exception of plasma cells, contained the same cell types found in vivo. The ultrastructural characteristics of the cells in vivo and in vitro were similar. In culture there were few large primary tumor cells. Macrophages, often involved in active phagocytosis, were prominent in the original tumor and were the most common cell type found in vitro. A tumor-associated immune response is postulated in this neoplasm.
在组织块和组织培养中研究了松果体生殖细胞瘤的精细结构。原始肿瘤包含大的原发性肿瘤细胞、淋巴细胞、巨噬细胞、浆细胞和星形胶质细胞。大的原发性肿瘤细胞的特征是细胞核大、细胞器相对较少、糖原丰富以及存在连接复合体。该肿瘤在体外生长良好，除浆细胞外，含有与体内发现的相同细胞类型。体内和体外细胞的超微结构特征相似。在培养物中，大的原发性肿瘤细胞很少。巨噬细胞常参与活跃的吞噬作用，在原始肿瘤中很突出，是体外发现的最常见细胞类型。推测该肿瘤存在肿瘤相关免疫反应。
