Liu Yan-Ming, Wang Cheng-Quan, Mu Hai-Bei, Cao Jun-Tao, Zheng Yan-Li
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, PR China.
Electrophoresis. 2007 Jun;28(12):1937-41. doi: 10.1002/elps.200600741.
A rapid and sensitive method to detect three catecholamines, isoprenaline, epinephrine, and dopamine, by CE coupled with direct luminol-potassium periodate chemiluminescence (CL) detection is described. The conditions for CE separation and CL reaction were systematically optimized. Under the optimum conditions, the baseline separation of three catecholamines was achieved within 6.5 min. The LODs obtained in standard solution were 5.3 x 10(-8 )mol/L for isoprenaline, 4.7 x 10(-8 )mol/L for epinephrine, and 1.5 x 10(-7 )mol/L for dopamine. The RSD of the migration time and peak area were less than 1.8 and 3.6% (n = 5), respectively. The present method was applied to the determination of the dopamine in urine samples of cigarette smokers and nonsmokers. The results obtained indicate that there is a close relationship between the content of dopamine in human urine and the amount of cigarettes smoked daily; the level of dopamine in smokers is higher than in nonsmokers.
描述了一种通过毛细管电泳（CE）结合直接鲁米诺-高碘酸钾化学发光（CL）检测来检测三种儿茶酚胺（异丙肾上腺素、肾上腺素和多巴胺）的快速灵敏方法。对CE分离和CL反应条件进行了系统优化。在最佳条件下，6.5分钟内实现了三种儿茶酚胺的基线分离。在标准溶液中获得的检测限分别为：异丙肾上腺素5.3×10⁻⁸ mol/L、肾上腺素4.7×10⁻⁸ mol/L、多巴胺1.5×10⁻⁷ mol/L。迁移时间和峰面积的相对标准偏差分别小于1.8%和3.6%（n = 5）。该方法应用于吸烟者和非吸烟者尿液样本中多巴胺的测定。所得结果表明，人尿液中多巴胺的含量与每日吸烟量密切相关；吸烟者体内多巴胺水平高于非吸烟者。
