从草药中发现治疗阿尔茨海默病的药物：抵御β-淀粉样蛋白（1-42）损伤的神经保护作用
Alzheimer's disease drug discovery from herbs: neuroprotectivity from beta-amyloid (1-42) insult.
作者信息
Kim Darrick S H L, Kim Jin-Yung, Han Ye Sun
机构信息
CurXceL Corporation, The Business and Technology Center, West Lafayette, IN 47906,
出版信息
J Altern Complement Med. 2007 Apr;13(3):333-40. doi: 10.1089/acm.2006.6107.
OBJECTIVE
To comparatively evaluate selected herbs for their ability to protect neuronal cells from direct betaA(1-42) insult.
DESIGN
Twenty-seven (27) herbs were selected, extracted with aqueous methanol (90%) and chloroform, and the extracts were evaluated for their ability to protect PC12 rat pheochromocytoma and primary neuronal cells from betaA(1-42) insult using both 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction assay and lactate dehydrogenase efflux assay.
RESULTS
Curcuma aromatia (ul-keum) and Zingiber officinale (ginger) extracts effectively protected cells from betaA(1-42) insult, followed by Ginkgo biloba (ginkgo), Polygonatum sp. (King Solomon's seal), Cinnamum cassia (Chinese cinnamon), Rheum coreanum (Korean rhubarb), Gastrodia elata (gastrodia), and Scutellaria baicalensis (skullcap). Several extracts showed cytotoxicity at high concentration (approximately 150 microg/mL), whereas other extracts did not at all protect cells from betaA(1-42) insult.
CONCLUSION
Selective herbs may be potentially important resources to discover drug candidates against the onset of Alzheimer's disease.
目的
比较评估所选草药保护神经元细胞免受β淀粉样蛋白（1-42）直接损伤的能力。
设计
选取27种草药，用90%甲醇水溶液和氯仿进行提取，通过3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐还原试验和乳酸脱氢酶外排试验，评估提取物保护PC12大鼠嗜铬细胞瘤细胞和原代神经元细胞免受β淀粉样蛋白（1-42）损伤的能力。
结果
莪术（ul-keum）和生姜提取物能有效保护细胞免受β淀粉样蛋白（1-42）损伤，其次是银杏（ginkgo）、黄精属植物（玉竹）、肉桂（中国肉桂）、朝鲜大黄（Korean rhubarb）、天麻（gastrodia）和黄芩（skullcap）。几种提取物在高浓度（约150微克/毫升）时显示出细胞毒性，而其他提取物根本不能保护细胞免受β淀粉样蛋白（1-42）损伤。
结论
选择性草药可能是发现抗阿尔茨海默病发病候选药物的潜在重要资源。
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