Wepsic H T, Nickel R, Alaimo J
Cancer Res. 1976 Jan;36(1):246-50.
The growth properties of single-tumor-cell suspensions prepared by enzymatic digestion of solid tumors from Morris hepatomas 7777, 5123tc, and 3924a and the presence of tumor-specific transplantation antigen for tumor lines 7777 and 3924a were described. Two of the tumor cell lines (7777 and 3924a) showed consistent i.m. tumor growth following the inoculation of 1 x 10(5) tumor cells, and a similar dose of 5123tc tumor cells resulted in inconsistent tumor growth. Two of the tumor lines (5123tc and 7777) were associated with rapid appearance of lung metastases, whereas with line 3924a metastatic lung lesions rarely developed despite its rapid i.m. tumor growth rate. Tumor resistance to rechallenge with a threshold inoculum of tumor cells was present in approximately 15 to 50% of the animals following amputation of an existing tumor mass. Resistance to a challenge tumor cell inoculum could also be accomplished by immunization with irradiated tumor cells. Tumor-specific resistance was demonstrated to tumor line 3924a in that "immune" animals were able to resist a challenge with 3924a tumor cells but did not resist a challenge with tumor line 9098.
描述了通过酶消化Morris肝癌7777、5123tc和3924a的实体瘤制备的单肿瘤细胞悬液的生长特性，以及肿瘤系7777和3924a的肿瘤特异性移植抗原的存在情况。其中两个肿瘤细胞系（7777和3924a）在接种1×10⁵个肿瘤细胞后，肌内肿瘤生长一致，而相同剂量的5123tc肿瘤细胞则导致肿瘤生长不一致。其中两个肿瘤系（5123tc和7777）与肺转移的快速出现相关，而3924a系尽管其肌内肿瘤生长速度很快，但很少发生肺转移病变。在切除现有肿瘤块后，约15%至50%的动物对阈值接种量的肿瘤细胞再次攻击具有肿瘤抗性。用照射过的肿瘤细胞进行免疫也可以实现对攻击肿瘤细胞接种的抗性。对3924a肿瘤系表现出肿瘤特异性抗性，即“免疫”动物能够抵抗3924a肿瘤细胞的攻击，但不能抵抗9098肿瘤系的攻击。
