Slamani Mohamed-Adel, Krol Andrzej, Beaumont Jacques, Price Robert L, Coman Ioana L, Lipson Edward D
ITT Industries, Advanced Engineering and Sciences, Alexandria, VA 22315, USA.
Microsc Microanal. 2006 Apr;12(2):106-12. doi: 10.1017/S143192760606017X.
We have implemented and tested a new automatic method for the montage synthesis and three-dimensional (3D) reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce "tiles" of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated.
我们已经实现并测试了一种新的自动方法，用于从共聚焦激光扫描显微镜数据（CLSM）中进行大组织体积的蒙太奇合成和三维（3D）重建。该方法依赖于相邻图像之间相位相关性的最大化。它在一个大型标本（小鼠心脏）上进行了测试，该标本被切成多个厚度适合CLSM的单独切片。这些切片进行了水平（平面内）和垂直（垂直于光学平面）扫描，以生成3D体积的“切片”。相位相关性最大化应用于平面内切片的蒙太奇合成以及给定物理切片内光学切片的3D对齐。对新方法的性能进行了评估。
