Andong National University, Andong, Korea.
Nutr Cancer. 2010;62(8):1113-9. doi: 10.1080/01635581.2010.515529.
Lunasin, a unique 43-amino acid peptide found in a number of seeds, has been shown to be chemopreventive in mammalian cells and in a skin cancer mouse model. To elucidate the role of cereals in cancer prevention, we report here the prevalence, bioavailability, and bioactivity of lunasin from barley. Lunasin is present in all cultivars of barley analyzed. The liver and kidney of rats fed with lunasin-enriched barley (LEB) show the presence of lunasin in Western blot. Lunasin extracted from the kidney and liver inhibits the activities of HATs (histone acetyl transferases), yGCN5 by 20% and 18% at 100 nM, and PCAF activity by 25% and 24% at 100 nM, confirming that the peptide is intact and bioactive. Purified barley lunasin localizes in the nuclei of NIH 3T3 cells. Barley lunasin added to NIH 3T3 cells in the presence of the chemical carcinogen MCA activates the expression of tumor suppressors p21 and p15 by 45% and 47%, decreases cyclin D1 by 98%, and inhibits Rb hyperphosphorylation by 45% compared with the MCA treatment alone. We conclude that lunasin is prevalent in barley, bioavailable, and bioactive and that consumption of barley could play an important role of cancer prevention in barley-consuming populations.
Lunasin 是一种在许多种子中发现的独特的 43 个氨基酸肽，已被证明在哺乳动物细胞和皮肤癌小鼠模型中具有化学预防作用。为了阐明谷物在癌症预防中的作用，我们在这里报告了大麦中 lunasin 的流行率、生物利用度和生物活性。在所分析的所有大麦品种中都存在 lunasin。用富含 lunasin 的大麦（LEB）喂养的大鼠的肝脏和肾脏在 Western blot 中显示出 lunasin 的存在。从肾脏和肝脏中提取的 lunasin 抑制 HATs（组蛋白乙酰转移酶）的活性，在 100 nM 时对 yGCN5 的抑制率分别为 20%和 18%，对 PCAF 活性的抑制率分别为 25%和 24%，证实该肽是完整的且具有生物活性。纯化的大麦 lunasin 定位于 NIH 3T3 细胞的核内。在存在化学致癌剂 MCA 的情况下，添加到 NIH 3T3 细胞中的大麦 lunasin 可使肿瘤抑制因子 p21 和 p15 的表达分别增加 45%和 47%，使 cyclin D1 减少 98%，并使 Rb 过度磷酸化减少 45%，与 MCA 单独处理相比。我们得出的结论是，lunasin 在大麦中普遍存在，具有生物利用度和生物活性，并且大麦的消费可能在食用大麦的人群中发挥重要的癌症预防作用。
