共价固定化重组人白细胞介素-1受体拮抗剂-弹性蛋白样多肽融合蛋白对脂多糖刺激的人单核细胞炎症谱的影响
The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes.
作者信息
Kim Dong-Hwan, Smith Jason T, Chilkoti Ashutosh, Reichert William M
机构信息
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
出版信息
Biomaterials. 2007 Aug;28(23):3369-77. doi: 10.1016/j.biomaterials.2007.04.010. Epub 2007 Apr 12.
Abstract
The objective of this research was to investigate whether immobilized anti-inflammatory cytokines will signal changes in the inflammatory profile of cultured monocytes. A fusion protein of recombinant human IL-1 receptor antagonist and elastin-like peptide (IL-1ra-ELP) was expressed in Escherichia coli. THP-1 human monocytes were cultured on either carboxyl-terminated self-assembled monolayers (SAMs), or SAMs with either covalently immobilized or soluble IL-1ra-ELP. LPS-stimulated monocytes exposed to either soluble or immobilized IL-1ra-ELP were prevented from cell differentiation, showed attenuated expression of pro-inflammatory cytokines, and had increased production of anti-inflammatory and pro-wound healing cytokines. These results suggest that immobilized anti-inflammatory cytokines have the potential to be immunomodulatory biomaterials.
摘要
本研究的目的是调查固定化抗炎细胞因子是否会引发培养单核细胞炎症谱的变化。重组人白细胞介素-1受体拮抗剂与弹性蛋白样肽的融合蛋白（IL-1ra-ELP）在大肠杆菌中表达。THP-1人单核细胞在羧基末端自组装单分子层（SAMs）上培养，或者在共价固定化或可溶性IL-1ra-ELP的SAMs上培养。暴露于可溶性或固定化IL-1ra-ELP的脂多糖刺激单核细胞可防止细胞分化，显示促炎细胞因子表达减弱，并增加抗炎和促进伤口愈合细胞因子的产生。这些结果表明，固定化抗炎细胞因子有潜力成为免疫调节生物材料。
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