McErlean P, Shackelton L A, Lambert S B, Nissen M D, Sloots T P, Mackay I M
Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Queensland, Australia.
J Clin Virol. 2007 Jun;39(2):67-75. doi: 10.1016/j.jcv.2007.03.012. Epub 2007 May 7.
Human rhinoviruses (HRVs) are some of the earliest identified and most commonly detected viruses associated with acute respiratory tract infections (ARTIs) and yet the molecular epidemiology and genomic variation of individual serotypes remains undefined.
To molecularly characterise a novel HRV and determine its prevalence and clinical impact on a predominantly paediatric population.
Nucleotide sequencing was employed to determine the complete HRV-QPM coding sequence. Two novel real-time RT-PCR diagnostic assays were designed and employed to retrospectively screen a well-defined population of 1244 specimen extracts to identify the prevalence of HRV-QPM during 2003.
Phylogenetic studies of complete coding sequences defined HRV-QPM as a novel member the genus Rhinovirus residing within the previously described HRV-A2 sub-lineage. Investigation of the relatively short VP1 sequence suggest that the virus is resistant to Pleconaril, setting it apart from the HRV A species. Sixteen additional HRV-QPM strains were detected (1.4% of specimens) often as the sole micro-organism present among infants with suspected bronchiolitis. HRV-QPM was also detected in Europe during 2006, and a closely related virus circulated in the United States during 2004.
We present the molecular characterisation and preliminary clinical impact of a newly identified HRV along with sequences representing additional new HRVs.
人鼻病毒（HRV）是最早被鉴定出且最常检测到的与急性呼吸道感染（ARTI）相关的病毒之一，但各个血清型的分子流行病学和基因组变异仍不明确。
对一种新型HRV进行分子特征分析，并确定其在以儿童为主的人群中的流行情况及其临床影响。
采用核苷酸测序法确定完整的HRV-QPM编码序列。设计并应用两种新型实时逆转录聚合酶链反应（RT-PCR）诊断检测方法，对1244份标本提取物的明确人群进行回顾性筛查，以确定2003年期间HRV-QPM的流行情况。
对完整编码序列的系统发育研究将HRV-QPM定义为鼻病毒属的一个新成员，属于先前描述的HRV-A2亚谱系。对相对较短的VP1序列的研究表明，该病毒对普来可那立耐药，这使其与HRV A种有所不同。另外检测到16株HRV-QPM毒株（占标本的1.4%），通常是疑似细支气管炎婴儿中唯一存在的微生物。2006年在欧洲也检测到了HRV-QPM，2004年在美国有一种密切相关的病毒传播。
我们展示了一种新鉴定的HRV的分子特征及其初步临床影响，以及代表其他新HRV的序列。
