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[八株弯曲杆菌外膜蛋白的SDS-PAGE分析]

[Analysis of outer membrane proteins from eight strains of Campylobacter in SDS-PAGE].

作者信息

Diao J, Lin Z, Gao X, Zhang C

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1991 Sep;22(3):278-81.

PMID:1748411
Abstract

The outer membrane proteins (OMPs) from 8 strains of Campylobacter, including 5 reference strains and 3 local strains, were isolated and examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that the population distributions of molecular weight of OMPs from different species and sources were alike based on the Rank Sum Test analysis. The number of bands of major OMP was around 2-3, the molecular weight of which varied from 33-68 kd. Moreover, three C. jejuni local strains isolated from patients in China showed common characteristics of OMPs to the reference strains. It is suggested that the OMPs of Campylobacter are of a conservative and stable structure.

摘要

从8株弯曲杆菌(包括5株参考菌株和3株本地菌株)中分离出外膜蛋白(OMPs),并通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)进行检测。秩和检验分析结果表明,来自不同种类和来源的OMPs分子量的总体分布相似。主要OMP的条带数约为2-3条,其分子量在33-68 kd之间变化。此外,从中国患者中分离出的3株空肠弯曲杆菌本地菌株显示出与参考菌株OMPs的共同特征。提示弯曲杆菌的OMPs具有保守且稳定的结构。

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