Jin T, Penner J L
Department of Microbiology, Faculty of Medicine, Banting Institute, University of Toronto, Ontario, Canada.
J Clin Microbiol. 1988 Dec;26(12):2480-3. doi: 10.1128/jcm.26.12.2480-2483.1988.
Outer membrane proteins from 12 strains of Campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with rabbit antisera prepared against Formalin-treated cell suspensions of three of the strains. The 92.5-kilodalton protein was confirmed to be a minor outer membrane protein and exhibited serological heterogeneity. Serological specificities of the proteins, however, were found not to correlate with serotypic specificities of the strains, as demonstrated by slide agglutination tests with whole-cell suspensions. The results suggested that the 92.5-kilodalton proteins were not likely to be participating antigenic determinants in the immunological reactions that give rise to agglutination of live-cell suspensions in the slide test.
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法以及用针对三株空肠弯曲菌经福尔马林处理的细胞悬液制备的兔抗血清进行免疫印迹法，对12株空肠弯曲菌的外膜蛋白进行了检测。92.5千道尔顿的蛋白被确认为一种次要外膜蛋白，并表现出血清学异质性。然而，正如用全细胞悬液进行玻片凝集试验所表明的，这些蛋白的血清学特异性与菌株的血清型特异性并不相关。结果表明，在玻片试验中引起活细胞悬液凝集的免疫反应中，92.5千道尔顿的蛋白不太可能是参与其中的抗原决定簇。
