Kanashiro A, Kabeya L M, Martinello F, Turato W M, Paula F S, Polizello A C M, Uyemura S A, Lucisano-Valim Y M
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Pharmazie. 2007 Apr;62(4):295-8.
Atherosclerosis has been described as an inflammatory disease in which polymorphonuclear leukocytes (PMNLs) seem to be involved. These cells may induce atherosclerotic lesions by releasing reactive oxygen species (ROS) and a sort of pro-inflammatory mediators. In this study, the PMNL oxidative metabolic status of Golden Syrian hamsters fed a normal diet (ND), or a high-fat diet (10% coconut oil plus 0.2% cholesterol) supplemented (R-HCD) or not (HCD) with 0.1% (w/w) rutin was evaluated after 120 days of treatment. PMNL oxidative metabolism was assessed by whole blood luminol-enhanced chemiluminescence and 2',7'-dichlorofluorescein diacetate-dependent flow cytometry. The results obtained by both methods were similar and showed no significant changes in ROS generation by PMNLs in blood samples from HCD or R-HCD animals when compared to ND. Furthermore it was shown that rutin supplementation did not significantly affect plasma lipid and lipoprotein levels in the hypercholesterolemic animals characterized by significantly increased total plasma cholesterol, triglycerides and low- and high-density lipoprotein cholesterol levels. The results suggest that in this model atherosclerosis development is not related to circulating PMNL activation and rutin supplementation has no immunomodulatory or hypocholesterolemic effects.
动脉粥样硬化被描述为一种炎症性疾病，其中多形核白细胞（PMNLs）似乎参与其中。这些细胞可能通过释放活性氧（ROS）和一种促炎介质来诱导动脉粥样硬化病变。在本研究中，在治疗120天后，评估了喂食正常饮食（ND）、高脂饮食（10%椰子油加0.2%胆固醇）且补充（R-HCD）或不补充（HCD）0.1%（w/w）芦丁的金黄叙利亚仓鼠的PMNL氧化代谢状态。通过全血鲁米诺增强化学发光和2',7'-二氯荧光素二乙酸酯依赖的流式细胞术评估PMNL氧化代谢。两种方法获得的结果相似，与ND相比，HCD或R-HCD动物血液样本中PMNL产生的ROS没有显著变化。此外，研究表明，在以总血浆胆固醇、甘油三酯以及低密度和高密度脂蛋白胆固醇水平显著升高为特征的高胆固醇血症动物中，补充芦丁对血浆脂质和脂蛋白水平没有显著影响。结果表明，在该模型中，动脉粥样硬化的发展与循环PMNL激活无关，补充芦丁没有免疫调节或降胆固醇作用。
