反式白藜芦醇对高胆固醇血症大鼠血小板-中性粒细胞复合物形成及中性粒细胞爆发的影响。
The effect of trans-resveratrol on platelet-neutrophil complex formation and neutrophil burst in hypercholesterolemic rats.
作者信息
Göçmen Ayşe Yeşim, Burgucu Durmuş, Karadoğan Ihsan, Timurağaoğlu Ayşen, Gümüşlü Saadet
机构信息
Department of Biochemistry, Faculty of Medicine, Bozok University, Antalya, Turkey.
出版信息
Exp Clin Cardiol. 2013 Spring;18(2):e111-4.
Transresveratrol (t-resveratrol; 3,5,4'-trihydroxy-trans-stilbene) is a polyphenolic compound found in fresh grapes, grape juice and wine, and has been found to reduce the total cholesterol level in hypercholesterolemic rats. The objective of the present study was to assess the effects of t-resveratrol on platelet-neutrophil complex formation and neutrophil reactive oxygen species (ROS) status in control and hypercholesterolemic rats using a modified flow cytometric method. Rats (n=80) were divided into five groups (control, ethanol, resveratrol, hypercholesterolemic and resveratrol-administered hypercholesterolemic groups), comprising 16 animals per group. Serum levels of lipids and H2O2 were determined using commercially available kits, while platelet-neutrophil complex formation and neutrophil ROS status were determined using a modified flow cytometric method. Serum total cholesterol and low-density lipoprotein cholesterol levels were found to be increased and the high-density lipoprotein cholesterol level was found to be decreased in the HC group compared with the control group (P<0.001). Treatment of HC rats with t-resveratrol significantly lowered total cholesterol and low-density lipoprotein cholesterol levels (P<0.001). In the hypercholesterolemic group, levels of serum H2O2 platelet-neutrophil complex formation and neutrophil ROS status were significantly increased (P<0.001). On the other hand, in the resveratrol-administered hypercholesterolemic group, serum H2O2 levels, platelet-neutrophil complex formation and neutrophil ROS status were decreased compared with the hypercholesterolemic group (P<0.001). Serum H2O2 levels, platelet-neutrophil complex and neutrophil ROS status were positively correlated with one another. The present study is the first to demonstrate the protective effect of t-resveratrol against hypercholesterolemia-induced platelet-neutrophil complex formation and neutrophil ROS burst. Further investigations on its plausible role in antihypercholesterolemic treatment are warranted.
反式白藜芦醇（t-白藜芦醇；3,5,4'-三羟基反式芪）是一种存在于新鲜葡萄、葡萄汁和葡萄酒中的多酚类化合物，已发现其可降低高胆固醇血症大鼠的总胆固醇水平。本研究的目的是使用改良的流式细胞术方法评估t-白藜芦醇对正常大鼠和高胆固醇血症大鼠血小板-中性粒细胞复合物形成及中性粒细胞活性氧（ROS）状态的影响。将大鼠（n = 80）分为五组（正常对照组、乙醇组、白藜芦醇组、高胆固醇血症组和给予白藜芦醇的高胆固醇血症组），每组16只动物。使用市售试剂盒测定血脂和H2O2的血清水平，同时使用改良的流式细胞术方法测定血小板-中性粒细胞复合物形成及中性粒细胞ROS状态。与对照组相比，高胆固醇血症组血清总胆固醇和低密度脂蛋白胆固醇水平升高，高密度脂蛋白胆固醇水平降低（P < 0.001）。用t-白藜芦醇治疗高胆固醇血症大鼠可显著降低总胆固醇和低密度脂蛋白胆固醇水平（P < 0.001）。在高胆固醇血症组中，血清H2O2、血小板-中性粒细胞复合物形成及中性粒细胞ROS状态水平显著升高（P < 0.001）。另一方面，在给予白藜芦醇的高胆固醇血症组中，与高胆固醇血症组相比，血清H2O2水平、血小板-中性粒细胞复合物形成及中性粒细胞ROS状态降低（P < 0.001）。血清H2O2水平、血小板-中性粒细胞复合物及中性粒细胞ROS状态彼此呈正相关。本研究首次证明了t-白藜芦醇对高胆固醇血症诱导的血小板-中性粒细胞复合物形成及中性粒细胞ROS爆发具有保护作用。有必要对其在抗高胆固醇血症治疗中可能的作用进行进一步研究。
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