Sedlak Kamil, Bartova Eva, Machova Jirina
Department of Virology and Serology, State Veterinary Institute Prague, Sidlistni 136/24, 165 03 Prague 6, Czech Republic.
J Wildl Dis. 2008 Jul;44(3):777-80. doi: 10.7589/0090-3558-44.3.777.
Blood samples were collected from wild boar (Sus scrofa) shot during the hunting season from 1999 to 2005 in the Czech Republic. Sera were tested by enzyme-linked immunosorbent assay for the presence of antibodies against classical swine fever virus (CSFV), swine vesicular disease virus (SVDV), Aujeszky's disease virus (ADV), and bovine viral diarrhea virus (BVDV). Indirect fluorescence antibody test was used for detection of antibodies against porcine circovirus type 2 (PCV-2) and transmissible gastroenteritis virus (TGEV). Antibodies against ADV, BVDV, PCV-2, and TGEV were detected in 30% (101 of 338), 1% (2 of 352), 43% (57 of 134), and 1% (1 of 134) of wild boars, respectively. Sera of 6,471 and 362 tested wild boars were negative for the presence of antibodies against CSFV and SVDV, respectively. This is the first survey of TGEV antibodies in wild boars and the first serologic survey of viral diseases in wild boars in the Czech Republic. Wild boars in the Czech Republic may act as a potential reservoir of ADV and thus have a role in the epidemiology of this disease.
1999年至2005年狩猎季节期间，在捷克共和国采集了被射杀的野猪（Sus scrofa）的血样。通过酶联免疫吸附测定法检测血清中是否存在针对经典猪瘟病毒（CSFV）、猪水疱病病毒（SVDV）、奥耶斯基氏病病毒（ADV）和牛病毒性腹泻病毒（BVDV）的抗体。采用间接荧光抗体试验检测针对2型猪圆环病毒（PCV - 2）和传染性胃肠炎病毒（TGEV）的抗体。分别在30%（338头中的101头）、1%（352头中的2头）、43%（134头中的57头）和1%（134头中的1头）的野猪中检测到针对ADV、BVDV、PCV - 2和TGEV的抗体。6471头和362头经检测的野猪血清分别对CSFV和SVDV抗体呈阴性。这是对野猪中TGEV抗体的首次调查，也是捷克共和国对野猪病毒性疾病的首次血清学调查。捷克共和国的野猪可能是ADV的潜在宿主，因此在该疾病的流行病学中具有一定作用。
