Ma Li, Zheng Li Wu, Cheung Lim Kwong
Discipline of Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong.
Front Biosci. 2007 May 1;12:3256-62. doi: 10.2741/2309.
Nicotine is the main chemical component in tobacco products and its effect on bone healing remains controversial. Distraction osteogenesis is an endogenous tissue engineering technique which provides an excellent platform to study bone healing and regeneration. This study aims to evaluate the dose dependent effect of nicotine on bone regeneration using a rabbit model of mandibular distraction osteogenesis. Twenty New Zealand white rabbits were randomly assigned to four groups: sham control, placebo control, low dose nicotine (0.75 g) and high dose nicotine (1.5 g). 60-day time release nicotine pellets or placebo pellets were implanted in the neck subcutaneous tissue of the rabbits one week before osteotomy was performed. Then after three day latency, eleven day active distraction and four week consolidation, the animals were sacrificed and subjected to examinations by radiography, micro-computed tomography and histological analysis. The significantly lower bone volume and appearance of chondrocytes in the high dose nicotine group indicated that the bone regeneration of distraction osteogenesis was compromised by high dose nicotine exposure.
尼古丁是烟草制品中的主要化学成分，其对骨愈合的影响仍存在争议。牵张成骨是一种内源性组织工程技术，为研究骨愈合和再生提供了一个极佳的平台。本研究旨在使用下颌骨牵张成骨的兔模型评估尼古丁对骨再生的剂量依赖性影响。将20只新西兰白兔随机分为四组：假手术对照组、安慰剂对照组、低剂量尼古丁组（0.75克）和高剂量尼古丁组（1.5克）。在进行截骨术前一周，将60天缓释尼古丁丸剂或安慰剂丸剂植入兔颈部皮下组织。然后在三天的延迟期、十一天的主动牵张期和四周的巩固期后，处死动物并进行X线摄影、显微计算机断层扫描和组织学分析。高剂量尼古丁组骨体积显著降低且出现软骨细胞，表明高剂量尼古丁暴露会损害牵张成骨的骨再生。
