Zheng M H, Papadimitriou J M, Nicholson G C
Department of Pathology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands.
Histochem J. 1991 Apr;23(4):180-8. doi: 10.1007/BF01046589.
Quantitative cytochemical, immunocytochemical, autoradiographic and electron cytochemical investigations have been used to compare osteoclasts with multinucleate giant cells that had been freshly obtained from the same animal. The levels of beta-acid galactosidase activity, the DNA in individual nuclei and the cellular protein content were similar in both cell types. However, osteoclasts generally possessed greater acid phosphatase and NADH dehydrogenase activity but lower levels of fluoride-inhibited non-specific esterase activity than multinucleate giant cells. The acid phosphatase activity in multinucleate giant cells was completely inhibited by 100 mM tartrate, but in osteoclasts only a 20% reduction in activity was observed. Formation of multinucleate giant cells in a "bone microenvironment" (thin bone slices) did not increase their content of tartrate-resistant acid phosphatase activity. Moreover, in osteoclasts, endogenous peroxidase activity was undetectable but present in several granules within the cytoplasm of multinucleate giant cells. Osteoclasts and multinucleate giant cells displayed a similar microtubules distribution, but calcitonin, which induced rearrangement of microtubules and cellular contraction in osteoclasts, had no effect on multinucleate giant cells. Thus, these investigations reveal both similarities and differences between these two syncytia and support the hypothesis that osteoclasts and multinucleate giant cells are related. Possibly osteoclasts arise from monocyte progenitors before commitment to a macrophage lineage has occurred.
已采用定量细胞化学、免疫细胞化学、放射自显影和电子细胞化学研究方法，将破骨细胞与从同一动物新鲜获取的多核巨细胞进行比较。两种细胞类型中β-酸性半乳糖苷酶活性水平、单个细胞核中的DNA以及细胞蛋白质含量相似。然而，与多核巨细胞相比，破骨细胞通常具有更高的酸性磷酸酶和NADH脱氢酶活性，但氟抑制的非特异性酯酶活性水平较低。多核巨细胞中的酸性磷酸酶活性被100 mM酒石酸盐完全抑制，但在破骨细胞中仅观察到活性降低20%。在“骨微环境”（薄骨切片）中多核巨细胞的形成并未增加其耐酒石酸酸性磷酸酶活性的含量。此外，在破骨细胞中未检测到内源性过氧化物酶活性，但在多核巨细胞细胞质内的几个颗粒中存在。破骨细胞和多核巨细胞显示出相似的微管分布，但降钙素可诱导破骨细胞中微管重排和细胞收缩，对多核巨细胞却没有影响。因此，这些研究揭示了这两种多核细胞之间的异同，并支持破骨细胞与多核巨细胞相关的假说。破骨细胞可能在单核祖细胞尚未定向分化为巨噬细胞谱系之前就已产生。
