Kasana Ramesh C, Sharma Upendra K, Sharma Nandini, Sinha Arun K
Hill Area Tea Science Division, Institute of Himalayan Bioresource Technology, Post Box No. 6, Palampur, 176061, Himachal Pradesh, India.
Curr Microbiol. 2007 Jun;54(6):457-61. doi: 10.1007/s00284-006-0627-z. Epub 2007 May 8.
Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacterium capable of converting isoeugenol to vanillin was isolated by conventional enrichment process from soils of Ocimum field. On the basis of morphological and physiochemical characteristics and 16S rRNA gene sequence analysis, the isolate was identified as Pseudomonas chlororaphis CDAE5 (EMBL # AM158279). Vanillin formation was analyzed by gas chromatography (GC), and its structure was confirmed by GC-mass spectrometry and nuclear magnetic resonance. After 24-h reaction, the vanillin concentration reached 1.2 g L(-1) from 10 g L(-1) isoeugenol in 20-mL reaction solution at 25 degrees C and 180 rpm. The strain showed potential to be a good candidate for biotechnological production of vanillin from isoeugenol. Further studies for standardization and optimization for higher yield of vanillin production needs to be investigated.
香兰素无疑是世界上最受欢迎且应用最广泛的调味剂之一。鉴于全球对天然香兰素的需求及其供应有限，包括生物转化在内的其生产替代路线正在不断探索。在这方面，通过常规富集方法从罗勒田土壤中分离出一种能够将异丁香酚转化为香兰素的新型土壤细菌。基于形态学、理化特性和16S rRNA基因序列分析，该分离株被鉴定为绿针假单胞菌CDAE5（EMBL编号：AM158279）。通过气相色谱（GC）分析香兰素的形成，并通过GC-质谱联用和核磁共振确认其结构。在25℃和180 rpm条件下，20 mL反应溶液中，10 g L(-1)异丁香酚经过24小时反应后，香兰素浓度达到1.2 g L(-1)。该菌株显示出从异丁香酚生物技术生产香兰素的良好潜力。需要进一步研究以实现香兰素生产更高产量的标准化和优化。
