Furukawa Hirotaka, Morita Hiroshi, Yoshida Toyokazu, Nagasawa Toru
Department of Biomolecular Science, Gifu University, Yanagido, Gifu 501-1193, Japan; Chisso Corporation, Yokohama Research Center, Kanazawa-ku, Yokohama 236-8605, Japan.
J Biosci Bioeng. 2003;96(4):401-3. doi: 10.1016/S1389-1723(03)90145-9.
Pseudomonas putida I58 was isolated from soil by a conventional enrichment culture method using isoeugenol as a sole carbon source. The strain utilized isoeugenol, vanillin and vanillic acid as carbon sources. On the other hand, the intermediates of the eugenol-degrading pathway, such as eugenol, coniferyl alcohol, coniferyl aldehyde and ferulic acid, were not utilized by this strain, indicating that isoeugenol is directly degraded to vanillin without the formation of ferulic acid. The resting cells of P. putida I58 rapidly converted isoeugenol into vanillic acid via vanillin with a conversion yield of 98% by 40-min incubation.
恶臭假单胞菌I58是通过一种传统的富集培养方法从土壤中分离出来的，该方法使用异丁香酚作为唯一碳源。该菌株利用异丁香酚、香草醛和香草酸作为碳源。另一方面，丁香酚降解途径的中间产物，如丁香酚、松柏醇、松柏醛和阿魏酸，该菌株不能利用，这表明异丁香酚直接降解为香草醛，而不形成阿魏酸。恶臭假单胞菌I58的静止细胞在40分钟的培养后，能迅速将异丁香酚通过香草醛转化为香草酸，转化率为98%。
