Unno Tatsuya, Kim Soo-Jin, Kanaly Robert A, Ahn Joong-Hoon, Kang Su-Il, Hur Hor-Gil
Department of Environmental Science and Engineering and International Environmental Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.
J Agric Food Chem. 2007 Oct 17;55(21):8556-61. doi: 10.1021/jf0715929. Epub 2007 Sep 15.
Newly isolated soil bacterium strain Jin1 was able to grow on both eugenol and isoeugenol each as sole source of carbon and energy. Based on bacterial 16S rDNA analysis, Jin1 belongs to Pseudomonas nitroreducens with a similarity of 98.92% (14/1297). P. nitroreducens Jin1 was found to biotransform eugenol and isoeugenol to vanillin by different pathways. Eugenol was biotransformed to vanillin through coniferyl alcohol and ferulic acid similarly to the pathway shown previously by Pseudomonassp. HR199 and vanillin produced from eugenol was rapidly metabolized to vanillic acid. Contrastively, Pseudomonas nitroreducens Jin1 did not appear to produce metabolic intermediates during the biotransformation of isoeugenol to vanillin which was finally biotransformed to vanillic acid with much slower rate. These results indicate that there seems to be different metabolic regulation systems for the biotransformation of eugenol and isoeugenol by this bacterium. Herein, we report on Pseudomonas nitroreducens Jin1, a novel bacterium that produces vanillin from eugenol and isoeugenol by two different metabolic pathways.
新分离出的土壤细菌菌株Jin1能够以丁香酚和异丁香酚分别作为唯一碳源和能源生长。基于细菌16S rDNA分析，Jin1属于硝基还原假单胞菌，相似度为98.92%（14/1297）。发现硝基还原假单胞菌Jin1通过不同途径将丁香酚和异丁香酚生物转化为香草醛。丁香酚通过松柏醇和阿魏酸生物转化为香草醛，这与之前假单胞菌属HR199所示途径相似，且由丁香酚产生的香草醛会迅速代谢为香草酸。相比之下，硝基还原假单胞菌Jin1在将异丁香酚生物转化为香草醛的过程中似乎不会产生代谢中间体，最终异丁香酚生物转化为香草酸的速率要慢得多。这些结果表明，该细菌对丁香酚和异丁香酚的生物转化似乎存在不同的代谢调控系统。在此，我们报道了硝基还原假单胞菌Jin1，一种通过两种不同代谢途径从丁香酚和异丁香酚产生香草醛的新型细菌。
