An immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: to Bacillus and beyond.

作者信息

Ruelle Virginie, Falisse-Poirrier Nandini, Elmoualij Benaissa, Zorzi Danièle, Pierard Olivier, Heinen Ernst, De Pauw Edwin, Zorzi Willy

机构信息

Center of Research on Prion Proteins, University of Liège, B-4000 Liège, Belgium.

出版信息

J Proteome Res. 2007 Jun;6(6):2168-75. doi: 10.1021/pr060661g. Epub 2007 May 9.

We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry (i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model.