Chen T-L, Siu L-K, Wu R C-C, Shaio M-F, Huang L-Y, Fung C-P, Lee C-M, Cho W-L
Section of General Medicine, Department of Medicine, Taipei Veterans General Hospital, and Institute of Tropical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
Clin Microbiol Infect. 2007 Aug;13(8):801-6. doi: 10.1111/j.1469-0691.2007.01744.x. Epub 2007 May 4.
Acinetobacter baumannii has emerged as a serious cause of nosocomial infections. Rapid identification of this pathogen is required so that appropriate therapy can be given and outbreaks controlled. This study evaluated a multiplex PCR and an automated ribotyping system for the rapid identification of Acinetobacter baumannii. In total, 22 different reference strains and 138 clinical isolates of Acinetobacter spp., identified by 16S-23S rRNA intergenic spacer (ITS) sequence analysis, were evaluated. All A. baumannii isolates (82 clinical isolates and one reference strain) were identified by the multiplex PCR method (specificity 100%). The sensitivity and specificity of the ribotyping system for identification of A. baumannii were 85.5% (71/83) and 93.5% (72/77), respectively. An additional 100 clinical isolates belonging to the Acinetobacter calcoaceticus-A. baumannii complex were used to compare these two methods for identification of A. baumannii, and this comparison revealed a level of disagreement of 14% (14 isolates). The accuracy of the multiplex PCR was 100%, which was confirmed by sequence analysis of the ITS and recA gene of these isolates. Thus, the multiplex PCR method dramatically increased the efficiency and speed of A. baumannii identification.
鲍曼不动杆菌已成为医院感染的一个严重病因。需要快速鉴定这种病原体，以便能够给予适当治疗并控制疫情暴发。本研究评估了一种多重PCR和一种自动化核糖体分型系统用于快速鉴定鲍曼不动杆菌。总共对通过16S - 23S rRNA基因间隔区（ITS）序列分析鉴定的22种不同参考菌株和138株不动杆菌属临床分离株进行了评估。所有鲍曼不动杆菌分离株（82株临床分离株和1株参考菌株）通过多重PCR方法得以鉴定（特异性为100%）。核糖体分型系统鉴定鲍曼不动杆菌的敏感性和特异性分别为85.5%（71/83）和93.5%（72/77）。另外100株属于醋酸钙不动杆菌 - 鲍曼不动杆菌复合体的临床分离株用于比较这两种鉴定鲍曼不动杆菌的方法，该比较显示不一致率为14%（14株分离株）。这些分离株的ITS和recA基因的序列分析证实了多重PCR的准确性为100%。因此，多重PCR方法显著提高了鲍曼不动杆菌鉴定的效率和速度。
