Higgins P G, Wisplinghoff H, Krut O, Seifert H
Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.
Clin Microbiol Infect. 2007 Dec;13(12):1199-201. doi: 10.1111/j.1469-0691.2007.01819.x. Epub 2007 Sep 10.
A new PCR-based method that exploits differences in gyrB gene sequences was developed to distinguish between Acinetobacter baumannii and Acinetobacter genomic sp. 13TU. Among 118 clinical and reference Acinetobacter strains, 102 of which were previously speciated by amplified rDNA restriction analysis as belonging to the Acinetobacter calcoaceticus-A. baumannii complex, the method correctly identified 31 A. baumannii and 54 Acinetobacter genomic sp. 13TU isolates to the species level. The method was rapid, specific and easy to interpret.
开发了一种基于聚合酶链反应（PCR）的新方法，该方法利用gyrB基因序列的差异来区分鲍曼不动杆菌和不动杆菌基因组种13TU。在118株临床和参考不动杆菌菌株中，其中102株先前通过扩增核糖体DNA限制性分析被鉴定为属于醋酸钙不动杆菌-鲍曼不动杆菌复合体，该方法在种水平上正确鉴定出31株鲍曼不动杆菌和54株不动杆菌基因组种13TU分离株。该方法快速、特异且易于解读。
