Albitar Lina, Pickett Gavin, Morgan Marilee, Davies Suzy, Leslie Kimberly K
Obstetrics and Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, The Cancer Research and Treatment Center, The University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA.
Gynecol Oncol. 2007 Jul;106(1):52-64. doi: 10.1016/j.ygyno.2007.02.033. Epub 2007 May 8.
Endometrial cancer models are critical to the advancement of investigation, and Ishikawa H and Hec50co cells have been used as research tools. The purpose of these studies is to verify the degree to which these commonly used cell models share the molecular characteristics of the two major in vivo endometrial cancer subtypes, I and II.
The studies reported include an analysis of pathologic features, tumor suppressor mutations, detailed karyotyping, and cell cycle regulation.
Ishikawa H cells are hormone responsive and have lost PTEN expression. In addition they have lost RB1 expression due to a deletion in exon 9. Hec50co cells have lost p53 expression due to a deletion at the junction of exon 6 and intron 6-7. Compared to Ishikawa H cells, Hec50co cells harbor many more chromosomal rearrangements (29 versus seven), and the doubling time is more rapid. The percent of cells in each phase of the cell cycle is reported and linked to cell cycle regulators.
We present extensive data indicating that Ishikawa H cells are excellent models for type I endometrial cancers, and Hec50co cells faithfully replicate the molecular characteristics of type II endometrial cancers. These studies allow testing of new therapeutic regimens using appropriate cell models.
子宫内膜癌模型对于研究进展至关重要，石川H细胞和Hec50co细胞已被用作研究工具。这些研究的目的是验证这些常用细胞模型在多大程度上具有两种主要的体内子宫内膜癌亚型（I型和II型）的分子特征。
所报道的研究包括病理特征分析、肿瘤抑制基因突变分析、详细的核型分析以及细胞周期调控分析。
石川H细胞对激素有反应，且PTEN表达缺失。此外，由于外显子9缺失，它们还失去了RB1表达。Hec50co细胞因外显子6与内含子6 - 7交界处的缺失而失去了p53表达。与石川H细胞相比，Hec50co细胞有更多的染色体重排（29处对7处），且倍增时间更快。报告了细胞周期各阶段的细胞百分比，并将其与细胞周期调节因子联系起来。
我们提供了大量数据表明石川H细胞是I型子宫内膜癌的优秀模型，而Hec50co细胞忠实地复制了II型子宫内膜癌的分子特征。这些研究使得能够使用合适的细胞模型测试新的治疗方案。
