Hao Chen, Ding Lin, Zhang Xueji, Ju Huangxian
MOE Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, People's Republic of China.
Anal Chem. 2007 Jun 15;79(12):4442-7. doi: 10.1021/ac062344z. Epub 2007 May 11.
A novel architecture was designed by combining the biocompatibility of chitosan (CS) and excellent conductivity of carbon nanofiber (CNF). The controllable electrodeposition of soluble CNF-doped CS colloidal solution formed a robust CNF-CS nanocomposite film with good biocompatibility for the immobilization and cytosensing of K562 cells on an electrode. The formed architecture was characterized using scanning electron microscopic, infrared spectrum, contact angle, and thermogravimetric analyses. The adhesion of K562 cells on the nanocomposite film-modified electrode could be followed with electrochemical impedance spectroscopy and cyclic voltammetry. The presence of CNF facilitated the electrochemical behavior of K562 cells. The impedance of electronic transduction was related to the amount of the adhered cells, producing a highly sensitive impedance sensor for K562 cells ranging from 5 x 10(3) to 5.0 x 10(7) cells mL-1 with a limit of detection of 1 x 10(3) cells mL-1. This work suggested a strategy to prepare a biocompatible and conductive interface for immobilization and electrochemical detection of cells and opened a way for the application of CNF in cytosensing.
通过结合壳聚糖（CS）的生物相容性和碳纳米纤维（CNF）的优异导电性，设计了一种新型结构。可溶性CNF掺杂的CS胶体溶液的可控电沉积形成了一种坚固的CNF-CS纳米复合膜，该膜具有良好的生物相容性，可用于在电极上固定K562细胞并进行细胞传感。使用扫描电子显微镜、红外光谱、接触角和热重分析对形成的结构进行了表征。K562细胞在纳米复合膜修饰电极上的粘附情况可以通过电化学阻抗谱和循环伏安法进行跟踪。CNF的存在促进了K562细胞的电化学行为。电子转导的阻抗与粘附细胞的数量有关，从而产生了一种对K562细胞高度敏感的阻抗传感器，检测范围为5×10³至5.0×10⁷个细胞/mL⁻¹，检测限为1×10³个细胞/mL⁻¹。这项工作提出了一种制备用于细胞固定和电化学检测的生物相容性和导电界面的策略，并为CNF在细胞传感中的应用开辟了道路。
