Institute of Microstructure Technology, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Angew Chem Int Ed Engl. 2021 Aug 23;60(35):19176-19182. doi: 10.1002/anie.202103585. Epub 2021 Jul 19.
Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi-layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by monitoring reagent and product concentrations in real-time. Simultaneous monitoring of an independent enzymatic process (Urs) together with a cooperative process (GOx + Cat) was achieved, with chemical conversion modulation of the GOx + Cat process demonstrated by varying the order in which the hydrogel was assembled.
微尺度的分隔化学反应在生物技术中很重要，但在这些小尺度上监测分子含量具有挑战性。为了解决这个挑战，我们将具有电化学功能的紧凑、可重构反应池与高分辨率 NMR 光谱学集成在一起。我们通过监测固定在多层壳聚糖水凝胶组件中化学性质不同的层内的酶的活性来证明该系统的运行。作为基准，我们通过实时监测试剂和产物浓度来观察脲酶（Urs）、过氧化氢酶（Cat）和葡萄糖氧化酶（GOx）的并行活性。通过改变水凝胶的组装顺序，实现了对独立酶促过程（Urs）和协同过程（GOx + Cat）的同时监测，并通过改变水凝胶的组装顺序来证明 GOx + Cat 过程的化学转化调节。
