Ding Lin, Hao Chen, Xue Yadong, Ju Huangxian
Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, China.
Biomacromolecules. 2007 Apr;8(4):1341-6. doi: 10.1021/bm061224y. Epub 2007 Mar 22.
A novel nanocomposites gel was prepared by neutralizing a designer nanocomposites solution of chitosan encapsulated gold nanoparticles formed by reducing in situ tetrachloroauric acid in chitosan. The bio-inspired gel was designed for immobilization and electrochemical study of cells and monitoring adhesion, proliferation, and apoptosis of cells on electrodes. Using K562 leukemia cells as a model, an impedance cell sensor was constructed. The methods for preparation of the gel and immobilization of cells were simple and "green". The nanocomposites gel showed improved immobilization capacity for cells and good biocompatibility for preserving the activity of immobilized living cells. The living cells immobilized on glassy carbon electrode exhibited an irreversible voltammetric response and increased the electron-transfer resistance with a good correlation to the logarithmic value of concentration ranging from 1.34 x 10(4) to 1.34 x 10(8) cells mL-1 with a limit of detection of 8.71 x 10(2) cells mL-1 at 10sigma. This work implied that the nanocomposites gel based on biopolymer and nanoparticles possessed potential applications for biosensing and provided a new avenue for electrochemical investigation of cell adhesion, proliferation, and apoptosis.
通过中和壳聚糖包裹金纳米颗粒的定制纳米复合材料溶液制备了一种新型纳米复合凝胶，该溶液是通过在壳聚糖中原位还原四氯金酸形成的。这种受生物启发的凝胶旨在用于细胞的固定化和电化学研究，以及监测电极上细胞的粘附、增殖和凋亡。以K562白血病细胞为模型，构建了一种阻抗细胞传感器。凝胶的制备方法和细胞固定化方法简单且“绿色”。纳米复合凝胶对细胞表现出更好的固定化能力，并且具有良好的生物相容性，能够保持固定化活细胞的活性。固定在玻碳电极上的活细胞表现出不可逆的伏安响应，并增加了电子转移电阻，与浓度对数在1.34×10⁴至1.34×10⁸个细胞 mL⁻¹范围内具有良好的相关性，在10σ时检测限为8.71×10²个细胞 mL⁻¹。这项工作表明，基于生物聚合物和纳米颗粒的纳米复合凝胶在生物传感方面具有潜在应用，并为细胞粘附、增殖和凋亡的电化学研究提供了一条新途径。
