Heine H G, Trinidad L, Selleck P, Lowther S
CSIRO Livestock Industries, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong Vic 3220, Australia.
Avian Dis. 2007 Mar;51(1 Suppl):370-2. doi: 10.1637/7587-040206R.1.
Highly pathogenic avian influenza (AI) H5N1 viruses have been spreading from Asia since late 2003. Early detection and classification are paramount for control of the disease because these viruses are lethal to birds and have caused fatalities in humans. Here, we described TaqMan reverse transcriptase-polymerase chain reaction assays for rapid detection of all AI viruses (influenza type A) and for identification of H5N1 of the Eurasian lineage. The assays were sensitive and quantitative over a 10(5)-10(6) linear range, detected all of the tested AI viruses, and enabled differentiation between H5 and H7 subtypes. These tests allow definitive confirmation of an AI virus as H5 within hours, which is crucial for rapid implementation of control measures in the event of an outbreak.
自2003年末以来，高致病性禽流感（AI）H5N1病毒一直在亚洲传播。早期检测和分类对于控制该疾病至关重要，因为这些病毒对鸟类具有致命性，并已导致人类死亡。在此，我们描述了用于快速检测所有禽流感病毒（甲型流感）以及鉴定欧亚谱系H5N1的TaqMan逆转录聚合酶链反应检测方法。这些检测方法在10⁵ - 10⁶线性范围内具有敏感性和定量性，能检测所有测试的禽流感病毒，并能够区分H5和H7亚型。这些检测可在数小时内明确确认禽流感病毒为H5，这对于在疫情爆发时迅速实施控制措施至关重要。
