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一种反向遗传学系统的开发，该系统能够拯救重组禽流感病毒A/土耳其/英格兰/50 - 92/91（H5N1）。

Development of a reverse genetics system enabling the rescue of recombinant avian influenza virus A/Turkey/England/50-92/91 (H5N1).

作者信息

Howard Wendy, Hayman Anna, Lackenby Angie, Whiteley Alison, Londt Brandon, Banks Jill, McCauley John, Barclay Wendy

机构信息

School of Biological Sciences, University of Reading, Berkshire RG6 6AJ, United Kingdom.

出版信息

Avian Dis. 2007 Mar;51(1 Suppl):393-5. doi: 10.1637/7645-050906R1.1.

DOI:10.1637/7645-050906R1.1
PMID:17494592
Abstract

We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.

摘要

我们之前描述了利用一个成熟的反向遗传学系统，从克隆的cDNA中产生重组人甲型流感病毒。在此，我们组装了一组质粒，以实现从cDNA完全恢复禽H5N1流感病毒A/土耳其/英格兰/50 - 92/91。该系统使我们能够在cDNA中引入突变或截短，以产生在特定选定基因中发生特异性改变的突变病毒。这些突变病毒随后可用于未来在鸡身上的发病机制研究，以及用于了解禽流感病毒在人类中的宿主范围限制的研究。

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