不稳定的聚合酶-核蛋白相互作用不是禽流感病毒聚合酶在人细胞中受限的原因。
Unstable polymerase-nucleoprotein interaction is not responsible for avian influenza virus polymerase restriction in human cells.
机构信息
Imperial College London, Faculty of Medicine, Division of Infectious Disease, London, United Kingdom.
出版信息
J Virol. 2013 Jan;87(2):1278-84. doi: 10.1128/JVI.02597-12. Epub 2012 Oct 31.
Abstract
Avian-origin influenza virus polymerase activity can be dramatically increased in human cells with the PB2 E627K mutation. Previously, others have proposed that this mutation increases the stability of the viral ribonucleoprotein complex (vRNP) measured by the interaction between PB2 and NP. However, we demonstrate here that a variety of PB2 adaptive mutations, including E627K, do not enhance the stability of the vRNP but rather increase the amount of replicated RNA that results in more PB2-NP coprecipitation.
摘要
禽类起源的流感病毒聚合酶活性在具有 PB2 E627K 突变的人类细胞中可以显著增加。此前，其他人提出，该突变通过 PB2 和 NP 之间的相互作用增加了病毒核糖核蛋白复合物（vRNP）的稳定性。然而，我们在这里证明，包括 E627K 在内的各种 PB2 适应性突变并不会增强 vRNP 的稳定性，而是会增加复制的 RNA 量，从而导致更多的 PB2-NP 共沉淀。
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本文引用的文献
1
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J Virol. 2013 Jan;87(1):384-94. doi: 10.1128/JVI.01633-12. Epub 2012 Oct 17.
2
Influenza polymerase activity correlates with the strength of interaction between nucleoprotein and PB2 through the host-specific residue K/E627.流感聚合酶活性与核蛋白和 PB2 之间通过宿主特异性残基 K/E627 的相互作用强度相关。
PLoS One. 2012;7(5):e36415. doi: 10.1371/journal.pone.0036415. Epub 2012 May 3.
3
Adaptive mutations in NEP compensate for defective H5N1 RNA replication in cultured human cells.适应性突变可补偿 H5N1 病毒 RNA 在培养的人细胞中的复制缺陷。
Nat Commun. 2012 May 1;3:802. doi: 10.1038/ncomms1804.
4
Host- and strain-specific regulation of influenza virus polymerase activity by interacting cellular proteins.宿主和株系特异性调节细胞蛋白相互作用对流感病毒聚合酶活性的影响。
mBio. 2011 Aug 16;2(4). doi: 10.1128/mBio.00151-11. Print 2011.
5
PA residues in the 2009 H1N1 pandemic influenza virus enhance avian influenza virus polymerase activity in mammalian cells.2009 年 H1N1 大流行流感病毒中的 PA 残基增强了哺乳动物细胞中的禽流感病毒聚合酶活性。
J Virol. 2011 Jul;85(14):7020-8. doi: 10.1128/JVI.00522-11. Epub 2011 May 11.
6
Influence of PB2 host-range determinants on the intranuclear mobility of the influenza A virus polymerase.宿主范围决定因素对流感 A 病毒聚合酶核内迁移的影响。
J Gen Virol. 2011 Jul;92(Pt 7):1650-1661. doi: 10.1099/vir.0.031492-0. Epub 2011 Apr 6.
7
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J Virol. 2011 Jan;85(1):357-65. doi: 10.1128/JVI.01694-10. Epub 2010 Oct 20.
8
Biological and structural characterization of a host-adapting amino acid in influenza virus.流感病毒宿主适应性氨基酸的生物学和结构特征。
PLoS Pathog. 2010 Aug 5;6(8):e1001034. doi: 10.1371/journal.ppat.1001034.
9
Evidence for avian and human host cell factors that affect the activity of influenza virus polymerase.流感病毒聚合酶活性的宿主细胞因子的研究进展：禽类和人类宿主细胞因子的影响。
J Virol. 2010 Oct;84(19):9978-86. doi: 10.1128/JVI.01134-10. Epub 2010 Jul 14.
10
Adaptation of pandemic H1N1 influenza viruses in mice.大流行性 H1N1 流感病毒在小鼠中的适应性。
J Virol. 2010 Sep;84(17):8607-16. doi: 10.1128/JVI.00159-10. Epub 2010 Jun 30.
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