Witort Ewa J, Pattarino Jacopo, Papucci Laura, Schiavone Nicola, Donnini Martino, Lapucci Andrea, Lulli Matteo, Lo Russo Giulia, Mori Andrea, Dini Mario, Capaccioli Sergio
Florence, Italy From the Departments of Experimental Pathology and Oncology, and Plastic Surgery, University of Florence.
Plast Reconstr Surg. 2007 Apr 1;119(4):1191-1199. doi: 10.1097/01.prs.0000258395.81926.d8.
Autologous fat transplantation (or lipofilling) is an excellent technique for correction of cosmetic defects. The success of the procedure relies strongly on the techniques of harvesting and transferring viable adipocytes. The purpose of this study was to evaluate effects of two harvesting methods and coenzyme Q10 on the viability and apoptotic death of adipocytes collected for autologous lipofilling.
Human adipose tissue from six patients was collected by Luer-Lok syringe according to Coleman's technique or by means of an aspirator with a 680-mmHg vacuum. Half of each sample collected using Coleman's technique was treated with 10 muM Coenzyme Q10, and the other half served as untreated control. Viability and apoptosis were assessed by immunoenzymatic, biochemical, and morphological methods.
The harvesting of adipose tissue by aspirator reduced the viability and increased apoptotic death significantly more than harvesting tissue using Coleman's technique. Biochemical and morphological analyses confirmed that treatment of adipose tissue with coenzyme Q10 reduced and even inhibited apoptotic death of harvested adipocytes.
Coenzyme Q10 can rescue adipocytes from stress-induced apoptotic death.
自体脂肪移植（或脂肪填充）是矫正美容缺陷的一项出色技术。该手术的成功很大程度上依赖于获取和移植存活脂肪细胞的技术。本研究的目的是评估两种获取方法以及辅酶Q10对用于自体脂肪填充所采集脂肪细胞的活力和凋亡死亡的影响。
根据科尔曼技术，用鲁尔锁注射器从6名患者身上采集人体脂肪组织，或借助一个产生680毫米汞柱真空度的抽吸器采集。使用科尔曼技术采集的每个样本的一半用10微摩尔辅酶Q10处理，另一半作为未处理的对照。通过免疫酶法、生化法和形态学方法评估活力和凋亡情况。
与使用科尔曼技术采集组织相比，用抽吸器采集脂肪组织显著降低了活力并增加了凋亡死亡。生化和形态学分析证实，用辅酶Q10处理脂肪组织可减少甚至抑制所采集脂肪细胞的凋亡死亡。
辅酶Q10可使脂肪细胞免受应激诱导的凋亡死亡。
