Zekri Abdel Rahman N, Bahnassy Abeer A, Seif-Eldin Walid M, Alam El-Din Hanaa M, Madbouly Maha S, Zidan Anoud Z, El-Hoshy Khaled, El-Ramly Amany, Abdel-Hamid Nayera A
The Department of Virology, Cairo University.
J Egypt Natl Canc Inst. 2006 Jun;18(2):117-24.
Human papilloma viruses (HPVs) are small DNA tumor viruses that infect epithelial tissues and cause warts. One of the viral genes responsible for HPV's oncogenic activity is E6 which is known to inactivate the cellular p53 tumor suppressor gene. We aim to detect the presence of HPV infection and its different types in human warts, and to identify the relation between HPV and p53 expression in skin and genital lesions.
We studied markers of HPV infection in overall of 30 patients (20 with common warts, and 10 with genital warts). Also, 30 normal skin samples were taken from each patient as a normal control. Detection of HPV was done using polymerase chain reaction (PCR), and HPV typing was performed using LiPA (Line immuno Probe Assay). In addition, all skin lesions were examined by immunohistochemistry for p53 expression.
In patients with common warts, HPV DNA was found in 4/20 (20%) of cases which was of HPV types 11, 31, 6, 33 (p=0.28). Also, P53 expression was found in 4/20 (20%) of cases (p=0.26). No single patient showed reactivity of both HPV and p53 expression. In patients with genital warts, however, HPV DNA was found in 6/10 (60%) of cases. Of these, 5 cases were positive for HPV type 6 and one case had HPV type 11. Three patients (30%) were positive for p53, and two of them (66%) were positive for both HPV and p53. In the normal skin control, 2/30 (6.6%) were positive for HPV DNA which were of types 5, and 31.
We conclude that; (1) Prevalence rate of HPV infection in warts is higher than those of normal control group, and Egyptian patients with genital warts had higher prevalence rate of HPV than those with common warts, (2) In Egypt, HPV types 6, and 11 are the most prevalent genotypes associated with genital warts and HPV types 6, 11, 31, and 33 are associated with common warts, (3) There was no definite relation between p53 expression and HPV detection, (4) Also, there was no association between the different HPV types and p53 detection in these non-cancerous lesions.
人乳头瘤病毒（HPV）是一种感染上皮组织并引发疣的小型DNA肿瘤病毒。HPV致癌活性所涉及的病毒基因之一是E6，已知其可使细胞中的p53肿瘤抑制基因失活。我们旨在检测人疣中HPV感染的存在及其不同类型，并确定HPV与皮肤及生殖器病变中p53表达之间的关系。
我们研究了总共30例患者（20例寻常疣患者和10例尖锐湿疣患者）的HPV感染标志物。此外，从每位患者身上采集30份正常皮肤样本作为正常对照。使用聚合酶链反应（PCR）检测HPV，并使用线性免疫探针分析（LiPA）进行HPV分型。另外，通过免疫组织化学检查所有皮肤病变的p53表达情况。
在寻常疣患者中，4/20（20%）的病例检测到HPV DNA，其HPV类型为11、31、6、33（p = 0.28）。同样，4/20（约20%）的病例检测到P53表达（p = 0.26）。没有单一患者同时显示HPV和p53表达阳性。然而，在尖锐湿疣患者中，6/10（60%）的病例检测到HPV DNA。其中，5例HPV 6型阳性，1例HPV 11型阳性。3例患者（30%）p53阳性，其中2例（66%）HPV和p53均为阳性。在正常皮肤对照中，2/30（6.6%）的样本HPV DNA阳性，其类型为5型和31型。
我们得出以下结论：（1）疣中HPV感染的患病率高于正常对照组，埃及尖锐湿疣患者的HPV患病率高于寻常疣患者；（2）在埃及，HPV 6型和11型是与尖锐湿疣相关的最常见基因型，HPV 6型、11型、31型和33型与寻常疣相关；（3）p53表达与HPV检测之间没有明确关系；（4）在这些非癌性病变中，不同HPV类型与p53检测之间也没有关联。
