Burguera-Pascu Margarita, Rodríguez-Archilla Alberto, Baca Pilar
Department of Oral Medicine, School of Dentistry, University of Granada, Granada, Spain.
J Trace Elem Med Biol. 2007;21(2):92-101. doi: 10.1016/j.jtemb.2006.12.003. Epub 2007 Feb 21.
The antimicrobial efficacy of zinc (Zn) salts (sulfate and acetate) against Streptococcus mutans (S. mutans) present in the oral cavity was tested in this study. The substantivity of Zn salts was assessed by determining the concentration of Zn in whole, unstimulated saliva and by measuring the magnitude of suppression of salivary S. mutans, 2h after rinsing. The concentration of Zn was measured by atomic absorption spectrometry (AAS) with electrothermal atomization (ET AAS) in saliva sampled before (basal) and 24h after mouth rinsing with different concentrations of Zn (0.1%, 0.5% and 1%) administrated as sulfate and acetate. The estimation of Zn levels in samples collected 30, 60, 90 and 120 min after rinsing was carried out by AAS with flame atomization (FAAS). Immediately after rinsing, the concentration of Zn in saliva sharply increased with respect to the baseline values (0.055+/-0.017 mg/L), followed by a sustained decrease, probably due to clearance of salivary flow or swallowing during sampling. A significant reduction (>87%) in the total mean S. mutans counts was found 2h after rinsing either with sulfate or acetate solutions, as evidence of the high substantivity and effectiveness of the Zn salts tested. A statistically significant inverse relationship (p<0.001 and the Pearson correlation coefficients between -34% and -50%) was found between Zn levels and the respective pH values measured in the samples collected 60 and 120 min after rinsing, sustaining the theory of bacterial glycolysis inhibition.
本研究测试了锌（Zn）盐（硫酸盐和醋酸盐）对口腔中变形链球菌（S. mutans）的抗菌效果。通过测定未刺激全唾液中的锌浓度以及测量漱口2小时后唾液中变形链球菌的抑制程度来评估锌盐的附着性。在用不同浓度（0.1%、0.5%和1%）的硫酸锌和醋酸锌漱口前（基础值）和漱口24小时后采集的唾液中，采用电热原子化原子吸收光谱法（ET AAS）测定锌浓度。在漱口后30、60、90和120分钟采集的样品中，通过火焰原子化原子吸收光谱法（FAAS）估算锌含量。漱口后，唾液中的锌浓度相对于基线值（0.055±0.017 mg/L）急剧增加，随后持续下降，这可能是由于采样期间唾液流动清除或吞咽所致。用硫酸盐或醋酸盐溶液漱口2小时后，变形链球菌总数平均显著降低（>87%），这证明了所测试锌盐的高附着性和有效性。在漱口后60和120分钟采集的样品中，锌含量与相应pH值之间存在统计学上显著的负相关关系（p<0.001，Pearson相关系数在-34%至-50%之间），支持了细菌糖酵解抑制理论。
