Dahlén G
Scand J Dent Res. 1984 Feb;92(1):38-42.
The antibacterial effects of chlorhexidine and an oxygen releasing agent on the salivary microflora were evaluated in healthy subjects in two separate experiments: 1) mouthrinsing on a single occasion and 2) mouthrinsing two or four times a day for 1 wk. Both experiments were performed in a cross-over study, and each subject was randomly tested for both rinsing agents and a saline control. The total bacterial number and some selected bacterial genera were followed in saliva samples taken prior to (zero-samples) and on three occasions after the rinsing or rinsing period. The reduction of the total bacterial number by mouthrinsing two or four times a day for 1 wk with chlorhexidine was statistically significant and lasted for over 4 h. After rinsing with chlorhexidine on one single occasion the reduction of the microflora was statistically significant and of the same magnitude as after rinsing two or four times daily for 1 wk. Although statistically significant reduction was obtained, the significant biologic effect of antimicrobial agents may be questioned. The number of Strep. mutans was dramatically reduced with chlorhexidine in some subjects and this reduction lasted for over 1 wk. The oxygen releasing agent did not reduce salivary flora significantly when rinsing took place two or four times daily for 1 wk.
在两项独立实验中，对健康受试者评估了洗必泰和一种氧释放剂对唾液微生物群的抗菌作用：1）单次漱口；2）每天漱口两次或四次，持续1周。两项实验均采用交叉研究进行，每个受试者都被随机测试两种漱口剂和一种生理盐水对照。在漱口或漱口期之前（零样本）以及之后的三个时间点采集唾液样本，跟踪总细菌数和一些选定的细菌属。用洗必泰每天漱口两次或四次，持续1周，总细菌数的减少具有统计学意义，且持续超过4小时。单次用洗必泰漱口后，微生物群的减少具有统计学意义，且与每天漱口两次或四次、持续1周后的减少幅度相同。尽管获得了具有统计学意义的减少，但抗菌剂的显著生物学效应可能受到质疑。在一些受试者中，变形链球菌的数量因洗必泰而大幅减少，且这种减少持续超过1周。当每天漱口两次或四次、持续1周时，氧释放剂并未显著减少唾液菌群。
