Abadi Marzieh Fattahi Dolat, Mehrabian Sedigheh, Asghari Babak, Namvar Amirmorteza Ebrahimzadeh, Ezzatifar Fatemeh, Lari Abdolaziz Rastegar
Department of Microbiology, Faculty of Biology Sciences, Branch of North Tehran, Islamic Azad University, Tehran, Iran.
GMS Hyg Infect Control. 2013 Apr 29;8(1):Doc05. doi: 10.3205/dgkh000205. eCollection 2013.
We developed an effective and non-irritant mouthwash that is alcohol-free and has a low concentration of silver nanoparticles (SNP) in order to be used for preventing oral cavity infections in immunocompromised oncologic patients. We studied antimicrobial effects of silver nanoparticles (SNP) in the range of (50-0.024 µg/ml) and 3% of ethanol (30,000 µg/ml) in mouthwash. Antimicrobial effects of two treatments were studied by doing challenge test on microorganisms such as Streptococcus mutans, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and measuring MIC and MBC (MFC) values of SNP toward mentioned microorganisms. These values of SNP respectively were in the range of (0.78-3.12) and (1.56-12.5 µg/ml). Results showed that SNP in the MIC and the lower concentrations killed all of the used microorganisms. No difference was observed between the antimicrobial effect of ethanol-free mouthwash containing SNP and mouthwash containing SNP and ethanol (30,000 µg/ml). SNP has high antimicrobial effects at low concentrations and it can be a good alternative for ethanol (30,000 µg/ml) because ethanol is also irritating, especially to sensitive or inflamed mucosa.
我们研发了一种有效且无刺激性的漱口水，它不含酒精且银纳米颗粒（SNP）浓度较低，用于预防免疫功能低下的肿瘤患者口腔感染。我们研究了漱口中银纳米颗粒（SNP）在（50 - 0.024微克/毫升）范围内以及3%乙醇（30,000微克/毫升）的抗菌效果。通过对变形链球菌、金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、白色念珠菌等微生物进行挑战试验，并测量SNP对上述微生物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC，对于真菌为最低杀菌浓度MFC）值，研究了两种处理的抗菌效果。SNP对这些微生物的这些值分别在（0.78 - 3.12）和（1.56 - 12.5微克/毫升）范围内。结果表明，MIC及更低浓度的SNP杀死了所有使用的微生物。含SNP的无乙醇漱口水与含SNP和乙醇（30,000微克/毫升）的漱口水在抗菌效果上未观察到差异。SNP在低浓度下具有高抗菌效果，并且它可以成为乙醇（30,000微克/毫升）的良好替代品，因为乙醇也具有刺激性，尤其是对敏感或发炎的黏膜。
