Savini G, Ronchi G F, Leone A, Ciarelli A, Migliaccio P, Franchi P, Mercante M T, Pini A
Department of Virology, OIE Reference Laboratory for Bluetonque, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise G. Caporale, Via Campo Boario, 64100 Teramo, Italy.
Vet Microbiol. 2007 Sep 20;124(1-2):140-6. doi: 10.1016/j.vetmic.2007.04.017. Epub 2007 Apr 8.
Because no suitable products are at the moment available to safely control the spread of BTV-16 in Europe, an inactivated vaccine was produced from the reference field isolate of bluetongue virus serotype 16. One group of six sheep was vaccinated subcutaneously with the inactivated vaccine twice, on days 0 and 28, whereas a second group of eight sheep was inoculated with saline solution and used as mock-vaccinated control animals. Seventy-eight days after the first vaccination, all sheep were inoculated subcutaneously with a suspension containing 10(6.3) TCID(50) of a virulent reference BTV-16 isolate. Apart from a transient inflammatory reaction at the injection site, no adverse effects were reported following vaccination. All vaccinated animals developed high titres (7.3-9.3log(2)(ED50%/50 microl)) of virus-specific neutralising antibodies and were resistant to challenge with BTV-16. Conversely, following challenge, control animals developed hyperthermia and long lasting high-titre viraemia.
由于目前没有合适的产品可用于安全控制蓝舌病病毒16型（BTV-16）在欧洲的传播，因此用蓝舌病病毒血清型16的参考田间分离株生产了一种灭活疫苗。一组6只绵羊在第0天和第28天皮下接种两次灭活疫苗，而另一组8只绵羊接种盐溶液并用作 mock 疫苗接种对照动物。首次接种疫苗78天后，所有绵羊皮下接种含有10(6.3) TCID(50) 的强毒参考BTV-16分离株的悬液。除注射部位出现短暂炎症反应外，接种疫苗后未报告有不良反应。所有接种疫苗的动物均产生了高滴度（7.3 - 9.3log(2)(ED50%/50微升)）的病毒特异性中和抗体，并对BTV-16攻击具有抗性。相反，在受到攻击后，对照动物出现体温过高和持续的高滴度病毒血症。
