Lee Myoung Woo, Moon Young Joon, Yang Mal Sook, Kim Sun Kyung, Jang In Keun, Eom Young-Woo, Park Joon Seong, Kim Hugh C, Song Kye Yong, Park Soon Cheol, Lim Hwan Sub, Kim Young Jin
Biomedical Research Institute, LifeCord Inc, Suwon, Republic of Korea.
Biochem Biophys Res Commun. 2007 Jun 29;358(2):637-43. doi: 10.1016/j.bbrc.2007.04.181. Epub 2007 May 7.
Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells, with practical and ethical advantages. To date, the presence of other stem cells in UCB remains to be established. We investigated whether other stem cells are present in cryopreserved UCB. Seeded mononuclear cells formed adherent colonized cells in optimized culture conditions. Over a 4- to 6-week culture period, colonized cells gradually developed into adherent mono-layer cells, which exhibited homogeneous fibroblast-like morphology and immunophenotypes, and were highly proliferative. Isolated cells were designated 'multipotent progenitor cells (MPCs)'. Under appropriate conditions for 2 weeks, MPCs differentiated into neural tissue-specific cell types, including neuron, astrocyte, and oligodendrocyte. Differentiated cells presented their respective markers, specifically, NF-L and NSE for neurons, GFAP for astrocytes, and myelin/oligodendrocyte for oligodendrocytes. In this study, we successfully isolated MPCs from cryopreserved UCB, which differentiated into the neural tissue-specific cell types. These findings suggest that cryopreserved human UCB is a useful alternative source of neural progenitor cells, such as MPCs, for experimental and therapeutic applications.
脐带血（UCB）是造血干细胞的丰富来源，具有实际和伦理优势。迄今为止，脐带血中其他干细胞的存在仍有待确定。我们研究了冷冻保存的脐带血中是否存在其他干细胞。接种的单核细胞在优化的培养条件下形成贴壁定植细胞。在4至6周的培养期内，定植细胞逐渐发育成贴壁单层细胞，其表现出均匀的成纤维细胞样形态和免疫表型，并且具有高度增殖性。分离出的细胞被命名为“多能祖细胞（MPCs）”。在适当条件下培养2周，多能祖细胞分化为神经组织特异性细胞类型，包括神经元、星形胶质细胞和少突胶质细胞。分化细胞呈现出各自的标志物，具体而言，神经元的NF-L和NSE、星形胶质细胞的GFAP以及少突胶质细胞的髓磷脂/少突胶质细胞。在本研究中，我们成功地从冷冻保存的脐带血中分离出多能祖细胞，其可分化为神经组织特异性细胞类型。这些发现表明，冷冻保存的人脐带血是用于实验和治疗应用的神经祖细胞（如多能祖细胞）的有用替代来源。
