聚合物在溶液中的内部力学响应。
Internal mechanical response of a polymer in solution.
作者信息
Cohen Adam E, Moerner W E
机构信息
Department of Physics, Stanford University, Stanford, California 94305, USA.
出版信息
Phys Rev Lett. 2007 Mar 16;98(11):116001. doi: 10.1103/PhysRevLett.98.116001. Epub 2007 Mar 14.
Abstract
We observed single molecules of fluorescently labeled double-stranded (ds) lambda DNA held in an anti-Brownian electrokinetic trap. From the measured density fluctuations we extract the density-density response function of the molecule over times >4.5 ms and distances >250 nm, i.e., how a perturbation in density in one part of the molecule propagates through the rest of the molecule. We find a nonmonotonic radial dependence of the relaxation time. In contrast with earlier measurements on freely diffusing dsDNA, we observe clear signs of internal hydrodynamic interactions.
摘要
我们观察了被捕获在反布朗电动阱中的荧光标记双链（ds）λDNA单分子。通过测量密度涨落，我们提取了分子在时间大于4.5毫秒、距离大于250纳米时的密度-密度响应函数，即分子某一部分的密度扰动如何在分子其余部分传播。我们发现弛豫时间具有非单调的径向依赖性。与早期对自由扩散dsDNA的测量结果不同，我们观察到了明显的内部流体动力学相互作用迹象。
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