Pardo B G, Fernández C, Hermida M, Vázquez-López A, Pérez M, Presa P, Calaza M, Alvarez-Dios J A, Comesaña A S, Raposo-Guillán J, Bouza C, Martínez P
Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Spain.
Genome. 2007 Mar;50(3):329-32. doi: 10.1139/g06-154.
The turbot is a flatfish species of great relevance to marine aquaculture in Europe. Only a limited number of microsatellites have been isolated to date in this species. To increase the number of potentially useful mapping markers, we screened simple sequence repeat (SSR)--enriched genomic libraries obtained from several di-, tri-, and tetranucleotide tandem repeat motifs. A total of 248 new polymorphic microsatellites were successfully optimized. The efficiency of the protocol applied (6.4%) was higher than that in other studies of fish that used the same method. Dinucleotide and perfect microsatellites were predominant in this species; the (AC)n motif was the most frequent class of repeat. Polymorphism and structural properties at these loci, together with 30 variable loci previously reported in turbot, were evaluated in 6 wild individuals. The number of alleles per locus ranged from 2 to 10, with an average of 4.046. The microsatellite markers characterized in this study will contribute to the development of the turbot genetic map, which can be used for quantitative trait locus (QTL) identification, marker-assisted selection programs, and other applications to improve its culture.
大菱鲆是一种对欧洲海水养殖具有重要意义的比目鱼。迄今为止，在该物种中仅分离出数量有限的微卫星。为了增加潜在有用的作图标记数量，我们筛选了从几个二核苷酸、三核苷酸和四核苷酸串联重复基序获得的富含简单序列重复（SSR）的基因组文库。总共成功优化了248个新的多态性微卫星。所应用方案的效率（6.4%）高于其他使用相同方法的鱼类研究。二核苷酸和完美微卫星在该物种中占主导地位；（AC）n基序是最常见的重复类别。在6个野生个体中评估了这些位点的多态性和结构特性，以及先前在大菱鲆中报道的30个可变位点。每个位点的等位基因数量从2到10不等，平均为4.046。本研究中鉴定的微卫星标记将有助于大菱鲆遗传图谱的构建，可用于数量性状位点（QTL）鉴定、标记辅助选择计划以及其他改善其养殖的应用。
