Bouza Carmen, Hermida Miguel, Pardo Belén G, Fernández Carlos, Fortes Gloria G, Castro Jaime, Sánchez Laura, Presa Pablo, Pérez Montse, Sanjuán Andrés, de Carlos Alejandro, Alvarez-Dios José Antonio, Ezcurra Susana, Cal Rosa M, Piferrer Francesc, Martínez Paulino
Department of Genetics, University of Santiago de Compostela, Lugo, Spain.
Genetics. 2007 Dec;177(4):2457-67. doi: 10.1534/genetics.107.075416.
A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked. The consensus map length was 1343.2 cM, with an average distance between markers of 6.5 +/- 0.5 cM. Similar length of female and male maps was evidenced. However, the mean recombination at common intervals throughout the genome revealed significant differences between sexes, approximately 1.6 times higher in the female than in the male. The comparison of turbot microsatellite flanking sequences against the Tetraodon nigroviridis genome revealed 55 significant matches, with a mean length of 102 bp and high sequence similarity (81-100%). The comparative mapping revealed significant syntenic regions among fish species. This study represents the first linkage map in the turbot, one of the most important flatfish in European aquaculture. This map will be suitable for QTL identification of productive traits in this species and for further evolutionary studies in fish and vertebrate species.
基于微卫星的大菱鲆（Scophthalmus maximus）共识连锁图谱由两个不相关的家系构建而成。作图群体来自一个由96个单倍体胚胎组成的雌核发育家系和一个由85个具有已知连锁相的全同胞后代组成的双亲二倍体家系。总共242个微卫星被定位到26个连锁群中，6个标记未连锁。共识图谱长度为1343.2厘摩，标记间平均距离为6.5±0.5厘摩。雌、雄图谱长度相似。然而，全基因组中常见区间的平均重组率显示两性间存在显著差异，雌性约为雄性的1.6倍。将大菱鲆微卫星侧翼序列与黑青斑河鲀基因组进行比较，发现55个显著匹配，平均长度为102 bp，序列相似性高（81 - 100%）。比较作图揭示了鱼类物种间显著的同线性区域。本研究构建了欧洲水产养殖中最重要的鲆鲽类之一——大菱鲆的首张连锁图谱。该图谱将适用于该物种生产性状的QTL鉴定以及鱼类和脊椎动物物种的进一步进化研究。
