高效液相色谱法同时测定委陵菜中七种黄酮类化合物的含量

Simultaneous determination of seven flavonoids in Potentilla multifida by HPLC.

作者信息

Xue Peifeng, Zhao Yuying, Wang Bin, Liang Hong

机构信息

Department of Natural Medicines, Peking University Health Science Center, Beijing 100083, PR China.

出版信息

J Chromatogr Sci. 2007 Apr;45(4):216-9. doi: 10.1093/chromsci/45.4.216.

DOI:10.1093/chromsci/45.4.216

PMID:17504571

Abstract

A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of seven flavonoids in Potentilla multifida: hyperin, quercetin-3-O-beta-D-glucopyranoside, luteolin-7-O-beta-D-glucuronide, apigenin-7-O-beta-D-glucuronide, quercetin, tribuloside, and apigenin. The method involves the use of a Hypersil octadecylsilyl silica (ODS) analytical column (125A, 5 microm, 4.6 x 250 mm) at 25 degrees C with the mixture of acetonitrile and aqueous H(3)PO(4) as the mobile phase and detection at 254 nm. The recovery of the method is 95.4-104.8%, and linearity (r > 0.9998) is obtained for all the flavonoids. The results indicate that the flavonoid content of P. multifida varied significantly from locality to locality.

摘要

描述了一种反相高效液相色谱法，用于同时测定多裂委陵菜中的七种黄酮类化合物：金丝桃苷、槲皮素 - 3 - O - β - D - 吡喃葡萄糖苷、木犀草素 - 7 - O - β - D - 葡萄糖醛酸苷、芹菜素 - 7 - O - β - D - 葡萄糖醛酸苷、槲皮素、刺蒺藜苷和芹菜素。该方法采用Hypersil十八烷基硅烷硅胶（ODS）分析柱（125Å，5μm，4.6×250mm），在25℃下，以乙腈和磷酸水溶液的混合物为流动相，并在254nm处进行检测。该方法的回收率为95.4 - 104.8%，所有黄酮类化合物均获得线性关系（r > 0.9998）。结果表明，多裂委陵菜中黄酮类化合物的含量因产地不同而有显著差异。