Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, Sakh'yanovoy str., 6, Ulan-Ude 670047, Russia.
Department of Biochemistry and Biotechnology, North-Eastern Federal University, 58 Belinsky Str., Yakutsk 677-027, Russia.
Molecules. 2014 Dec 24;20(1):224-48. doi: 10.3390/molecules20010224.
A chemical study of Potentilla anserina L. herb (Rosaceae) of Siberian origin led to the isolation of 17 compounds. Three ellagitannins-potentillin, agrimonic acid A and B-are reported for the first time in this species. With a view to rapid quantitative analysis, a new method was developed for simultaneous determination of major phenolic compounds in P. anserina, including caffeic acid, myricetin-3-O-glucuronide, agrimoniin, ellagic acid, miquelianin, isorhamnetin-3-O-glucuronide, and kaempferol-3-O-rhamnoside. The quantitative determination was conducted by microcolumn reversed phase high-performance liquid chromatography with UV detection. Separation was performed using a ProntoSIL-120-5-C18 AQ column (60 mm × 1 mm × 5 μm) with six-step gradient elution of aqueous 0.2 М LiClO4 in 0.006 M HClO4 and acetonitrile as mobile phases. The components were quantified by HPLC-UV at 270 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The reproducibility was evaluated by intra- and inter-day assays, and RSD values were less than 2.8%. The recoveries were between 97.15 and 102.38%. The limits of detection ranged from 0.21 to 1.94 μg/mL, and limits of quantification ranged from 0.65 to 5.88 μg/mL, respectively. Various solvents, extraction methods, temperatures, and times were evaluated to obtain the best extraction efficiency. The developed method was successfully applied for the analysis of selected pharmaceutical products: 12 batches of P. anserina herb collected from three Siberian regions (Yakutia, Buryatia, Irkutsk), two commercial samples of P. anserina herb, and some preparations (liquid extract, tincture, decoction, infusion, and dry extract).
对西伯利亚产委陵菜（蔷薇科）全草的化学研究导致分离出 17 种化合物。首次在该种中报道了三种鞣花单宁——鞣花酸、熊果酸 A 和 B。为了快速定量分析，开发了一种新方法，用于同时测定委陵菜中的主要酚类化合物，包括咖啡酸、杨梅素-3-O-葡萄糖苷、仙鹤草酚、鞣花酸、杨梅素、异鼠李素-3-O-葡萄糖苷和山奈酚-3-O-鼠李糖苷。定量测定采用微柱反相高效液相色谱法，用 UV 检测。采用 ProntoSIL-120-5-C18 AQ 柱（60mm×1mm×5μm），以 0.2M LiClO4 在 0.006M HClO4 中的水溶液和乙腈为流动相，进行六步梯度洗脱。用 HPLC-UV 在 270nm 下对各成分进行定量。所有校准曲线在测试范围内均呈良好线性（r2>0.999）。通过日内和日间试验评估了重现性，RSD 值小于 2.8%。回收率在 97.15%至 102.38%之间。检测限范围为 0.21-1.94μg/mL，定量限范围为 0.65-5.88μg/mL。评估了各种溶剂、提取方法、温度和时间，以获得最佳提取效率。所建立的方法成功应用于分析选定的药用产品：从西伯利亚三个地区（雅库特、布里亚特、伊尔库茨克）采集的 12 批委陵菜全草、两种商业样品的委陵菜全草以及一些制剂（液体提取物、酊剂、煎剂、浸剂和干提取物）。
