Lincoln Bryan, Schinkinger Stefan, Travis Kort, Wottawah Falk, Ebert Susanne, Sauer Frank, Guck Jochen
Institut für Experimentalphysik I, Universität Leipzig, Linnéstr. 5, 04103, Leipzig, Germany.
Biomed Microdevices. 2007 Oct;9(5):703-10. doi: 10.1007/s10544-007-9079-x.
A dual-beam fiber laser trap, termed the optical stretcher when used to deform objects, has been combined with a capillary-based microfluidic system in order to serially trap and deform biological cells. The design allows for control over the size and position of the trap relative to the flow channel. Data is recorded using video phase contrast microscopy and is subsequently analyzed using a custom edge fitting routine. This setup has been regularly used with measuring rates of 50-100 cells/h. One such experiment is presented to compare the distribution of deformability found within a normal epithelial cell line to that of a cancerous one. In general, this microfluidic optical stretcher can be used for the characterization of cells by their viscoelastic signature. Possible applications include the cytological diagnosis of cancer and the gentle and marker-free sorting of stem cells from heterogeneous populations for therapeutic cell-based approaches in regenerative medicine.
一种双光束光纤激光阱，在用于使物体变形时称为光学拉伸器，已与基于毛细管的微流控系统相结合，以便连续捕获和变形生物细胞。该设计允许控制阱相对于流动通道的大小和位置。使用视频相差显微镜记录数据，随后使用自定义边缘拟合程序进行分析。此设置经常以每小时50 - 100个细胞的测量速率使用。展示了一个这样的实验，以比较正常上皮细胞系和癌细胞系中发现的变形性分布。一般来说，这种微流控光学拉伸器可用于通过细胞的粘弹性特征对细胞进行表征。可能的应用包括癌症的细胞学诊断以及从异质群体中温和且无标记地分选干细胞，用于再生医学中基于细胞治疗的方法。
