Thirkill Twanda L, Cao Tim, Stout Michael, Blankenship Thomas N, Barakat Abdul, Douglas Gordon C
Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, CA 95616-8643, USA.
Biochim Biophys Acta. 2007 Jun;1773(6):1007-14. doi: 10.1016/j.bbamcr.2007.04.006. Epub 2007 Apr 20.
The factors that regulate trophoblast invasion of the uterine vasculature are incompletely understood. In this paper we show that macaque trophoblasts express the mucin, MUC1, and that it is involved in trophoblast-endothelial interaction. Immunocytochemistry, Western blotting and RT-PCR analyses confirmed that MUC1 was expressed by isolated early gestation macaque trophoblasts. MUC1 was also detected in endovascular trophoblasts in sections of placental-decidual tissue during early gestation. A blocking antibody against MUC1 reduced trophoblast adhesion to uterine endothelial cells and also blocked trophoblast transendothelial migration. MUC1 is known to bind to Intercellular Adhesion Molecule-1 (ICAM-1) in other systems. Incubation in the presence of a blocking antibody against Intercellular Adhesion Molecule-1 (ICAM-1) or recombinant ICAM-1 modestly, but significantly, reduced transendothelial trophoblast migration. These results are consistent with the idea that MUC1 is involved in trophoblast adhesion to uterine endothelial cells and in trophoblast transendothelial migration.
调节滋养层细胞侵入子宫血管的因素尚未完全明确。在本文中，我们发现猕猴滋养层细胞表达黏蛋白MUC1，且它参与滋养层细胞与内皮细胞的相互作用。免疫细胞化学、蛋白质印迹法和逆转录聚合酶链反应分析证实，分离出的妊娠早期猕猴滋养层细胞表达MUC1。在妊娠早期胎盘 - 蜕膜组织切片的血管内滋养层细胞中也检测到了MUC1。抗MUC1阻断抗体可降低滋养层细胞与子宫内皮细胞的黏附，并阻断滋养层细胞的跨内皮迁移。已知在其他系统中，MUC1可与细胞间黏附分子 -1（ICAM -1）结合。在抗细胞间黏附分子 -1（ICAM -1）阻断抗体或重组ICAM -1存在的情况下孵育，可适度但显著地减少滋养层细胞的跨内皮迁移。这些结果与MUC1参与滋养层细胞与子宫内皮细胞黏附及滋养层细胞跨内皮迁移的观点一致。
