Mishra N, Dubey R, Rajukumar K, Tosh C, Tiwari A, Pitale S S, Pradhan H K
High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462021, India.
Vet Microbiol. 2007 Oct 6;124(3-4):340-7. doi: 10.1016/j.vetmic.2007.04.023. Epub 2007 Apr 19.
Recent studies have shown that bovine viral diarrhea virus (BVDV) type 1 is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5' un-translated region (5' UTR), E(rns) structural coding region and NS5B nonstructural coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E(rns) and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep.
近期研究表明，1型牛病毒性腹泻病毒（BVDV）在印度牛群中广泛流行。在2004年至2006年期间对来自七个邦随机采集的562份血样进行的监测中，通过巢式逆转录聚合酶链反应（nRT-PCR）在两只印度本地山羊中检测到了2型BVDV。根据病毒中和试验以及与单克隆抗体的反应性，从它们身上分离出的病毒在抗原上被归类为BVDV 2。对三个不同基因组区域，即5'非翻译区（5'UTR）、E(rns)结构编码区和NS5B非结构编码区进行的系统发育分析将印度山羊分离株鉴定为BVDV 2a，与来自北美和欧洲的毒株具有密切相似性，这表明其可能是通过贸易引入的。它被置于2a分支内的一个单独进化枝中，在E(rns)和NS5B区域具有独特的突变。这是印度关于2型BVDV的首次报道，也是山羊物种中第二次有记录。从山羊中分离出BVDV 2需要对牛和羊进行强化监测。
