Matsuzaka Kenichi, Sato Daisuke, Ishihara Kazuyuki, Hashimoto Sadamitsu, Yoshinari Masao, Katakura Akira, Inoue Takashi
Oral Health Science Center HRC7, Tokyo Dental College, Chiba, Japan.
Bull Tokyo Dent Coll. 2006 Nov;47(4):167-70. doi: 10.2209/tdcpublication.47.167.
Defensins are known to play an important role in defense against bacteria. It is also known that immunity against infection is compromised with age. The purpose of this study was to evaluate the localization of human beta-defensin (HBD)-2 in human gingival epithelia according to age. Gingival epithelia in maxillary premolar buccal normal regions was immunohistochemically stained for HBD-2. Specimens were divided into two groups: 6 cases in a young group (<20 years old) and 7 cases in an elderly group (>50 years old). Expression of HBD-2 in gingival epithelium in young subjects was mostly detected in the superficial layer of the parakeratinized layer, while some areas of the spinous cell layer in elderly subjects were positive for HBD-2, as was the superficial layer. Two cases in the young group and 5 cases in the elderly group immunoreacted with HBD-2 in the spinous cell layer. Furthermore, immunoreaction was stronger in the elderly group. The results revealed HBD-2 positive cells in spinous cells in the elderly group and in the parakeratinized layer in the young group.
已知防御素在抵抗细菌方面发挥着重要作用。还已知抗感染免疫力会随着年龄增长而受损。本研究的目的是根据年龄评估人β-防御素（HBD）-2在人牙龈上皮中的定位。对上颌前磨牙颊侧正常区域的牙龈上皮进行HBD-2免疫组织化学染色。标本分为两组：年轻组（<20岁）6例，老年组（>50岁）7例。年轻受试者牙龈上皮中HBD-2的表达主要在不全角化层的表层检测到，而老年受试者棘细胞层的一些区域以及表层HBD-2呈阳性。年轻组有2例和老年组有5例在棘细胞层与HBD-2发生免疫反应。此外，老年组的免疫反应更强。结果显示老年组棘细胞和年轻组不全角化层中有HBD-2阳性细胞。
